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Insect gut metagenome

ABSTRACT: Pieris brassicae gut

PROVIDER: PRJNA555186 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11795900_1.fastq.gz	Fastqsanger.gz
	SRR11795900_2.fastq.gz	Fastqsanger.gz
	SRR11795901_1.fastq.gz	Fastqsanger.gz
	SRR11795901_2.fastq.gz	Fastqsanger.gz
	SRR11799803_1.fastq.gz	Fastqsanger.gz

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Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated different single or dual stresses: the larvae Pieris brassicae, egg extract of Pieris brassicae, the bacterial Xanthomonas campestris pv. raphani, the aphid Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae.

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Project description:Plants can cope with stress better if they experience a mild form of the stress before the actual \\"real\\" stress event. In Arabidopsis thaliana it is known that plants that harboured eggs of the White cabbage butterfly (Pieris brassicae) before larval feeding can defend better against the herbivore stress. The main aim of the experiment was to compare the priming effect induced by insect egg deposition of Pieris brassicae between vegetative and reproductive (first open flowers) Arabidopsis thaliana plants on the transcriptional level. We used a full factorial setup consisting of a) untreated control plants , b) plants which experienced eggs for 6 days without larval feeding after that period, c) plants which experienced no eggs before larval feeding for 24 hours d) plants which experienced eggs for 6 days and larval herbivory for 24 hours. This setup was conducted with 6 week old vegetative plants and 10 week old reproductive plants were the first flowers were open. For all treatments leaf tissue from the leaves that experienced egg oviposition and/or larval feeding were collected. From reproductive plants flower buds were collected as well.

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Gene expression in Arabidopsis thaliana leaves after chilling and/or herbivory by Pieris brassicae or Mamestra brassice larvae or after mechanical wounding

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