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Lactobacillus helveticus

ABSTRACT: Lactobacillus helveticus Raw sequence reads

PROVIDER: PRJNA555465 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR9866100_1.fastq.gz	Fastqsanger.gz
	SRR9866100_2.fastq.gz	Fastqsanger.gz
	SRR9866110_1.fastq.gz	Fastqsanger.gz
	SRR9866110_2.fastq.gz	Fastqsanger.gz
	SRR9866120_1.fastq.gz	Fastqsanger.gz

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Genome heterogeneity in Lactobacillus helveticus: Rethinking a “niche specialist”*

Project description:Lactobacillus helveticus is a rod-shaped lactic acid bacterium that is widely used in the manufacture of fermented dairy foods and for production of bioactive peptides from milk proteins. Although L. helveticus is commonly associated with milk environments, phylogenetic studies show it is closely related to an intestinal species, Lactobacillus acidophilus, which has been shown to impart probiotic health benefits to humans. This relationship has fueled a prevailing hypothesis that L. helveticus is a highly specialized derivative of L. acidophilus which has adapted to acidified whey. However, L. helveticus has also been sporadically recovered from non-dairy environments, which argues the species may not be as highly specialized as is widely believed. This study employed genome sequence analysis and comparative genome hybridizations to investigate genomic diversity among L. helveticus strains collected from cheese, whey, and whiskey malt, as well as commercial cultures used in manufacture of cheese or bioactive dairy foods. Results revealed considerable variability in gene content between some L. helveticus strains, and indicated the species should not be viewed as a strict dairy-niche specialist. In addition, comparative genomic analyses provided new insight on several industrially and ecologically important attributes of L. helveticus that may facilitate commercial strain selection.

2013-08-16 | GSE23660 | GEO

Genome heterogeneity in Lactobacillus helveticus: Rethinking a “niche specialist”*

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Project description:Lactobacillus helveticus Genome sequencing

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Project description:Lactobacillus helveticus Genome sequencing

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Transcriptional Profile of Lactobacillus helveticus grown in milk vs. MRS medium

Project description:L. helveticus is used to modulate cheese flavor and as a starter organism in certain cheese varieties. Our group has compiled a draft (4x) sequence for the 2.4 Mb genome of an industrial strain L. helveticus CNRZ32. The primary aim was to investigate expression of 168 completely sequenced genes during growth in milk and MRS medium using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays where the non-interrupted sequence contigs were covered by consecutive 24-mer probes. Keywords: growth conditions response

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Project description:Lactobacillus helveticus Genome sequencing and assembly

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