Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of transcriptional changes in CD103 positive dendritic cells in mice lacking Il33 expression in DCs relative to control mice

ABSTRACT: Comparison of transcriptional changes in CD103 positive dendritic cells in mice lacking Il33 expression in DCs relative to control mice

PROVIDER: PRJNA555625 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9719742.fastq.gz	Fastqsanger.gz
	SRR9719743.fastq.gz	Fastqsanger.gz
	SRR9719744.fastq.gz	Fastqsanger.gz
	SRR9719745.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Comparison of transcriptional changes in CD103 positive dendritic cells in mice lacking Il33 expression in DCs relative to control mice

Project description:IL33 is an alarmin protein that is known to be released by epithelial cells as a response to damage. There is also evidence that dendritic cells express IL33, however its function in these cells is not well understood. To investigate the distinct function of IL33 in dendritic cells, deep sequencing of mRNA transcripts was conducted on FACS sorted DCs in mice lacking Il33 expression in CD11c expressing cells compared to control mice.

2020-11-13 | GSE134581 | GEO

Activation and presentation of SIINFEKL:Kb by lung CD11b+ and CD103+ DCs after intranasal SIIN-Q11.

Project description:To investigate whether differentially expressed genes induced by SIIN-Q11 were shared between lung CD11b+ and CD103+ DCs, we performed gene expression profiling analysis of lung CD11b+ and CD103+ DCs after intranasal SIIN-Q11.

2023-11-29 | GSE216097 | GEO

Intranasal SIIN-Q11 induces shared transcriptional profiles in LN CD103+ and CD11b+ DCs

Project description:To gain insights into the activation of LN DCs by intranasal SIIN-Q11, we conducted a transcriptional analysis on flow-sorted LN CD11b+ and CD103+ DCs

2023-11-29 | GSE216098 | GEO

Induced CD103+ Dendritic Cells

Project description:Multiple subsets of FLT3L-dependent dendritic cells (DCs) control T cell tolerance and immunity. In mouse tissues, CD8α-like DCs are identified by CD103 expression. This DC subset efficiently enters lymph nodes and cross-presents antigens, rendering CD103+ DCs promising targets for therapeutic tolerance induction or vaccination. However, only limited numbers of CD103+ DCs can be isolated with current methods. Moreover, bone marrow cultures with FLT3L produce complex mixtures of DC subsets. We developed a novel method for generating large numbers of Batf3-dependent CD103+ DCs. We used microarray analysis to compare in vitro generated CD103+ and CD103- DCs and correlated their expression patterns to published profiles and signatures of DC subsets.

2014-12-31 | GSE54472 | GEO

Distinct murine mucosal Langerhans cell subsets develop from pre-DCs and monocytes

Project description:Langerhans cells (LCs) populate the mucosal epithelium, a major entry portal for pathogens, yet their ontogeny remains unclear. In contrast to skin LCs originating from self-renewing radioresistant embryonic precursors, we found that oral mucosal LCs derive from circulating radiosensitive precursors. Mucosal LCs can be segregated into CD103+CD11blow (CD103+LCs) and CD11b+CD103- (CD11b+LCs) subsets. We further demonstrated that similar to non-lymphoid dendritic cells (DCs), CD103+LCs originate from pre-DCs, whereas CD11b+LCs differentiate from both pre-DCs and monocytic precursors. Despite this ontogenetic discrepancy between skin and mucosal LCs, transcriptomic signature and immunological function of oral LCs highly resemble those of skin LCs but not DCs. These findings, along with their epithelial position, morphology and expression of LC-associated phenotype strongly suggest that oral mucosal LCs are genuine LCs. Collectively, in a tissue-dependent manner, murine LCs differentiate from at least three distinct precursors (embryonic, pre-DCs and monocytic) in steady state The following cells were isolated from mice (2-4 replicates): Lung DCs, mucosal CD103+ LC, mucosal CD11b+ LC, Skin LC. Transcriptome analysis was performed.

2015-08-31 | E-GEOD-68789 | biostudies-arrayexpress

RNA-seq analyses of two intestinal dendritic cell subsets, CD103+CD11b- and CD103-CD11b+, from wild type and CbfbF/F:CD11c-Cre mice.

Project description:We report phenotypes in gut dendritic cell development by loss of Runx/Cbfb transcription factor complexes. To further examine function of residual CD103+CD11b- and CD103-CD11b+ DCs, we performed RNA-seq analyses and compared gene expression signatures between control and Cbfb-deficient cells.

2019-08-01 | GSE130380 | GEO

Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal T cells via TGF-?

Project description:In comparison to murine dendritic cells (DCs), less is known about the function of human DCs in tissues. Here, we analyzed, using lung tissues from humans and humanized mice, the role of human CD1c+ and CD141+ DCs in determining the type of CD8+ T cell immunity to live-attenuated influenza virus (LAIV) vaccine. We found that both lung DC subsets acquired influenza antigens in vivo and expanded specific cytotoxic CD8+ T cells in vitro. However, lung-tissue-resident CD1c+ DCs but not CD141+ DCs were able to drive CD103 expression on CD8+ T cells and promote CD8+ T cell accumulation in lung epithelia in vitro and in vivo. CD1c+ DCs induction of CD103 expression was dependent on membrane-bound TGF-?1. Thus, CD1c+ and CD141+ DCs generate CD8+ T cells with different properties, and CD1c+ DCs specialize in the regulation of mucosal CD8+ T cells. Total RNA were isolated from purified human CD1c+ (BDCA1+) and CD141+ (BDCA3+) mDCs sorted from different tissues, including human blood, spleen and lungs of humanized mice, and human lungs. Eighteen samples in total were analyzed from different donors and tissues.

2013-04-04 | E-GEOD-43184 | biostudies-arrayexpress

Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal T cells via TGF-β

Project description:In comparison to murine dendritic cells (DCs), less is known about the function of human DCs in tissues. Here, we analyzed, using lung tissues from humans and humanized mice, the role of human CD1c+ and CD141+ DCs in determining the type of CD8+ T cell immunity to live-attenuated influenza virus (LAIV) vaccine. We found that both lung DC subsets acquired influenza antigens in vivo and expanded specific cytotoxic CD8+ T cells in vitro. However, lung-tissue-resident CD1c+ DCs but not CD141+ DCs were able to drive CD103 expression on CD8+ T cells and promote CD8+ T cell accumulation in lung epithelia in vitro and in vivo. CD1c+ DCs induction of CD103 expression was dependent on membrane-bound TGF-β1. Thus, CD1c+ and CD141+ DCs generate CD8+ T cells with different properties, and CD1c+ DCs specialize in the regulation of mucosal CD8+ T cells.

2013-04-04 | GSE43184 | GEO

Intranasal SIIN-Q11 induces shared transcriptional profiles in LN CD103+ and CD11b+ DCs

Project description:Intranasal SIIN-Q11 induces shared transcriptional profiles in LN CD103+ and CD11b+ DCs

| PRJNA892095 | ENA

Distinct murine mucosal Langerhans cell subsets develop from pre-DCs and monocytes

2015-08-31 | GSE68789 | GEO