Project description:Higher diagnostic value of metagenomic next-generation sequencing in acute infection than chronic infection: a multicenter retrospective study
| PRJNA1059147 | ENA
Project description:Diagnostic value of whole blood metagenomic next-generation sequencing in bloodstream infectious diseases
Project description:Purpose: The goals of this study are to compare Next-generation sequencing (NGS)-derived transcriptome profiling (RNA-seq) in the lung of three tyeps of mice during influenza infection. Methods: Total RNA from lung was extracted using a modified TRIzol protocol and spectrophometrically quantitated. Library preparation and sequencing were conducted using 3’ inTAG next-generation sequencing . Differential gene expression for day 6 post influenza infection was determined relative to mock inoculated mice. Results: Differentially expressed genes were defined using p-value <0.01 and FDR-corrected p-value <0.1 cutoffs. We identified the transcripts in the lung of RIG-I-/-, MAVS -/- mice during influenza infection Our study represents the first detailed analysis of lung transcriptomes of Wild Type , RIG-I-/-, MAVS -/- mice during influenza infection , with biologic replicates, generated by RNA-seq technology.
2018-11-28 | GSE114232 | GEO
Project description:Metagenomic next-generation sequence data of Suspected Infected Pancreatic Necrosis patients
| PRJNA771396 | ENA
Project description:The diagnostic performance of metagenomics next-generation sequencing in the suspected central nervous system infections
| PRJNA1118056 | ENA
Project description:Metagenomic next-generation sequencing for infection in immunocompromised children