Project description:We investigated changes in gene expression in rice plants exposed to RDV to gain some insight into the fundamental physiological and biochemical changes that are induced by viral infection, in the hope of finding clues that might help to control the viral disease. An analysis, using microarrays, of gene expression in rice plants infected with Rice dwarf virus revealed significant decreases in levels of expression of genes that are involved in the formation of cell walls, reflecting the stunted growth of diseased plants. The expression of plastid-related genes was also suppressed, as anticipated from the white chlorotic appearance of infected leaves. By contrast, the expression of defense- and stress-related genes was enhanced after viral infection. Keywords: disease state analysis
Project description:Common transcriptional responses of Arabidopsis thaliana protoplasts transfected with turnip crinkle virus (TCV) , hibiscus chlorotic ringspot virus (HCRSV) and their coat protein mutants.
Project description:In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt. By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation. Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected. Specifically, tomato adaptation resulted in a large set of constitutively down-regulated genes of unknown function in adapted mites compared to non-adapted mites. Additionally, upon tomato exposure, adapted mites exhibited an increased transcriptional plasticity of genes coding for detoxifying enzymes and xenobiotic transporters. Remarkably, adapted mites further exhibited a differential effect on host plant physiology compared to non-adapted mites. Adapted mites induced a greater chlorotic area on tomato leaves and triggered attenuated induced responses relative to those induced by non-adapted mites.
2015-07-21 | GSE68708 | GEO
Project description:First reports of Chrysanthemum virus B and Chrysanthemum chlorotic mottle viroid in South Africa