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Transcriptome analysis of freshly isolated young and aged rat oligodendrocyte progenitor cells (OPCs)

ABSTRACT: Transcriptome analysis of freshly isolated young and aged rat oligodendrocyte progenitor cells (OPCs)

PROVIDER: PRJNA556360 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9822273_1.fastq.gz	Fastqsanger.gz
	SRR9822273_2.fastq.gz	Fastqsanger.gz
	SRR9822274_1.fastq.gz	Fastqsanger.gz
	SRR9822274_2.fastq.gz	Fastqsanger.gz
	SRR9822275_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of freshly isolated young and aged rat oligodendrocyte progenitor cells (OPCs)

Project description:The age-related failure to regenerate lost oligodendrocytes from oligodendrocyte progenitor cells (OPCs) is associated with irreversible neurodegeneration in multiple sclerosis. Consequently, regenerative therapies for chronic demyelinating diseases remain a long sought after but elusive goal. Here we show that adult OPCs lose their inherent differentiation potential with increasing age. Moreover, aged OPCs do not remain responsive to pro-differentiation signals, posing significant constraints on the effectiveness of remyelination therapies based on the enhancement of OPC differentiation. We show that this reduced regenerative capacity is associated with hallmarks of cellular ageing, including reduced metabolic function and increased DNA damage. We find that fasting or treatment with metformin can reverse these changes and restore the regenerative capacity of aged OPCs, improving remyelination in aged animals following white matter injury. Importantly, aged OPCs treated with metformin regain their responsiveness to pro-differentiation factors, suggesting a potential for synergistic effects of rejuvenation and pro-differentiation therapies. Our findings have direct implications for understanding oligodendrocyte regeneration failure with ageing and how it might be reversed therapeutically.

2019-11-01 | GSE134765 | GEO

Changes in the oligodendrocyte cell progenitor cell proteome with ageing

Project description:Following CNS demyelination, adult oligodendrocyte progenitor cells (OPCs) can differentiate into new myelin-forming oligodendrocytes in a regenerative process called remyelination. While remyelination is very efficient in young adults, its efficiency declines progressively with ageing. Here we performed proteomic analysis on OPCs isolated acutely from the brains of neonate, young and aged female rats. Approximately 60% of the proteins are expressed at different levels in OPCs from neonates compared to their adult counterparts. The amount of myelin-associated and cell adhesion proteins are increased with age, while cholesterol-biosynthesis, transcription factors and cell cycle proteins decreased. Our experiments provide the first ageing OPC rodent proteome, revealing the distinct features of neonatal and adult OPCs, and providing new insights into why remyelination efficiency declines with ageing and potential roles for aged OPCs in other neurodegenerative diseases.

2022-02-25 | PXD010383 | Pride

Changes in the oligodendrocyte cell progenitor cell proteome with ageing

2020-05-22 | PXD013708 | Pride

Young CSF restores oligodendrogenesis and memory in aged mice via Fgf17

Project description:Recent understanding of how the systemic environment shapes the brain throughout life has led to numerous intervention strategies to slow brain ageing. Cerebrospinal fluid (CSF) makes up the immediate environment of brain cells, providing them with nourishing compounds. We discovered that infusing young CSF directly into aged brains improves memory function. Unbiased transcriptome analysis of the hippocampus identified oligodendrocytes to be most responsive to this rejuvenated CSF environment. We further showed that young CSF boosts oligodendrocyte progenitor cell (OPC) proliferation and differentiation in the aged hippocampus and in primary OPC cultures. Using SLAMseq to metabolically label nascent mRNA, we identified serum response factor (SRF), a transcription factor that drives actin cytoskeleton rearrangement, as a mediator of OPC proliferation following exposure to young CSF. With age, SRF expression decreases in hippocampal OPCs, and the pathway is induced by acute injection with young CSF. We screened for potential SRF activators in CSF and found that fibroblast growth factor 17 (Fgf17) infusion is sufficient to induce OPC proliferation and long-term memory consolidation in aged mice while Fgf17 blockade impairs cognition in young mice. These findings demonstrate the rejuvenating power of young CSF and identify Fgf17 as a key target to restore oligodendrocyte function in the ageing brain.

2022-05-11 | GSE198008 | GEO

Transcriptomic analysis of undifferentiated and differentiated NMR OPCs.

Project description:Transcriptome of long-lived naked-mole rat (NMR) oligodendrocyte progenitor cells (OPCs) were compared with OPCs of other species.

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DNA methylome sequencing of aged vs young AT2 cells

Project description:EM-Seq of DNA derived from freshly sorted AT2 cells from aged (24-30 months old) and young (3-4 months old) C57BL6/J mice

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Transcriptome analysis of freshly isolated young and aged mouse natural regulatory T cells

Project description:Transcriptome analysis of freshly isolated young and aged mouse natural regulatory T cells

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Gene expression analysis after GPR17 silencing in oligodendrocyte precursor cells (OPCs)

Project description:GPR17 silencing in OPCs accelerates their differentiation into fully mature oligodendrocytes. We performed a whole microarray profiling to identify altered signaling pathways after GPR17 silencing that are responsible for its effect on oligodendrocyte maturation.

2022-08-03 | GSE208409 | GEO

Gene expression analysis after miR-125a-3p over-expression in oligodendrocyte precursor cells (OPCs)

Project description:MiR-125a-3p over-expression in OPCs impairs their differentiation into fully mature oligodendrocytes. We performed a whole microarray profiling to identify new miR-125a-3p direct targets and altered signaling pathways responsible for its the detrimental effect on oligodendrocyte maturation.

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Project description:Freshly dissected Thymus, Kidney, and Spleen from young and aged mice were subjected to collagenase digestion to prepare single-cell suspension for the isolation of endothelial cells. Endothelial cells form the single-cell suspension was isolated using a magnetic bead-based separation method using CD144 and Endomucin antibody. RNA from 150,000 cells from each group was isolated using the RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer’s instructions. The library preparation was performed using QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina.

2021-01-23 | E-MTAB-8941 | biostudies-arrayexpress

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