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Impact of EPA 50uM on gene exprezsion in MC38 mouse colorectal cancer cells

ABSTRACT: Impact of EPA 50uM on gene exprezsion in MC38 mouse colorectal cancer cells

PROVIDER: PRJNA557255 | ENA |

REPOSITORIES: ENA

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Impact of EPA 50uM on gene exprezsion in MC38 mouse colorectal cancer cells

Project description:Genone wide differential gene expression analysis Identification of gene linked to mouse colorectal cancer cell response to EPA treatment through differential gene expression analysis in an EPA-sensitive cell line.

2019-07-30 | GSE135067 | GEO

Comparison of gene expression in EPA sensitive and EPA resistant mouse colorectal cancer cells

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Gene expression in colorectal liver metastasis tissues from EPA-treated patients

Project description:Eicosapentaenoic acid in its free fatty acid form (EPA-FFA), 2g daily, is safe and well-tolerated in patients undergoing liver resection surgery for colorectal liver metastasis.Oral EPA incorporates into colorectal liver metastasis tissue. EPA-FFA treatment is associated with reduced vascularity of liver metastases in Ï-3 PUFA-naÃ¯ve patients. Preoperative (median 30 days) EPA-FFA treatment may have prolonged benefit on postoperative overall and disease-free survival. We used whole genome expression array to study whether systemic CCL2 level changes were linked to a specific tumour gene expression profile in colorectal liver metastasis patients treated with EPA-FFA. 15 tumour RNA samples from colorectal liver metastasis patients treated with EPA-FFA during the EMT study (ClinicalTrials.gov NCT01070355) were extracted from formalin-fixed paraffin-wax embedded tissue blocks. The RNA samples were used for whole genome expression microarray experiments. We then performed a differential gene expression analysis to compare the tumour expression profile of patients with increased or decreased plasma CCL2 levels after intervention.

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