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Genome-wide high-throughput sequencing analysis of TLR4-overexpressing ovarian cancer cells treated with taxol and curcumin

ABSTRACT: Genome-wide high-throughput sequencing analysis of TLR4-overexpressing ovarian cancer cells treated with taxol and curcumin

PROVIDER: PRJNA558231 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9888022_1.fastq.gz	Fastqsanger.gz
	SRR9888022_2.fastq.gz	Fastqsanger.gz
	SRR9888023_1.fastq.gz	Fastqsanger.gz
	SRR9888023_2.fastq.gz	Fastqsanger.gz
	SRR9888024_1.fastq.gz	Fastqsanger.gz

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Genome-wide high-throughput sequencing analysis of TLR4-overexpressing ovarian cancer cells treated with taxol and curcumin

Project description:The purpose of this study is to approach overexpression of TLR4 which contributes the drug resistance in ovarian cancer cells. However, curcumin overcomes this effects. Comparison of the transcriptional regulated genes were performed: (1) analysis of TLR4-overexpressing (T4) and mock (MCS) SKOV3 cells by taxol treatment; (2) assay of taxol alone and combined with curcumin treatments in TLR4-overexpressing (T4) SKOV3 cells. Differential expression analysis was performed with ≥ 2-fold or ≤0.5-fold (p<0.05) changed expression for pathway enrichment analysis.

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Expression data from Caco-2 cells expressing TLR4 and associated mutants

Project description:Common missense mutations (D299G, T399I) have been recently identified in the human TLR4 gene. The aim of this study was to determine how TLR4 and associated mutants affect gene expression in Caco-2 cells. We used microarrays to asses gene expression profiles in Caco-2 stably overexpressing TLR4-WT, TLR4-D299G, TLR4-T399I or untransfected. Caco-2 clones stably overexpressing HA-tagged wildtype TLR4-WT, mutant TLR4-D299G or TLR4-T399I were generated. Prior to analysis, cell clones were cultured for 8 days in all experiments. RNA (triplicate) was extracted and hybridized on Affymetrix microarrays.

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Expression data from Caco-2 cells expressing TLR4 and associated mutants

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