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Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound

ABSTRACT: Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound

PROVIDER: PRJNA558262 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9888210_1.fastq.gz	Fastqsanger.gz
	SRR9888210_2.fastq.gz	Fastqsanger.gz
	SRR9888211_1.fastq.gz	Fastqsanger.gz
	SRR9888211_2.fastq.gz	Fastqsanger.gz
	SRR9888212_1.fastq.gz	Fastqsanger.gz

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Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound

Project description:As part of a wider project to assess the impact of ultrasound on in vitro plant growth, this paper aimed to determine whether the application of piezoelectric ultrasound (PE-US) would induce changes to the transcriptome of in vitro potato (Solanum tuberosum L.). After exposing explants (single-node segments with a single leaf) to PE-US (35 kHz; 70 W) for 20 min, the effect of this stressor was determined at 0 h, 24 h, 48 h, 1 w and 4 w to assess the possible immediate and residual effects of PE-US on the potato transcriptome.

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Transcriptomics of shade avoidance and drought in Solanum tuberosum.

Project description:The intent was to study, from transcriptome analysis, shade and drought responses in Solanum tuberosum (potato). We performed Illumina 50 bp single-end RNA-seq in tissues of control and treated var. Spunta wild-type plants. Drought experiments also included two independent AtBBX21-overexpressing (BBX21-OE) potato lines.

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Plant genes affected by Solanum tuberosum and Colorado potato beetle interaction

Project description:Potato leaves From Solanum tuberosum var. Kennebec will be wounded and oral secretions from 4th instar CPB will be isolated and added to the plants as described by Kruzmane et al (2002, Physiol. Plantarum 115:577-584). The leaf from the 6th node of the potato plant will be wounded or wounded and treated with oral secretions from CPB. Unwounded leaves from node 1-5 of the wounded and wounded plus oral secretions plants will be harvested as systemic material. The leaves will be harvested after 4 hrs and RNA will be isolated. 4 hrs was chosen because this represents a time when early and late induced genes should both be present. In addition, the leaf from the 6th node will be subjected to feeding by CPB that have been raised on potato leaves and starved for 16 hrs immediately prior to infestation. Insects will be allowed to feed for 1 hr and the leaves will be harvested after 3 additional hrs. An additional set of plants will be used to infest the leaf on the 6th node for 4 hrs. Leaves from the 6th node will be collected from uninfested plants after 4 hrs as a control. Three sets of 6-12 plants will be used for each sample. Keywords: Direct comparison

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mRNA transcription profile of potato (Solanum tuberosum L.) exposed to ultrasound during different stages of in vitro plantlet development

Project description:Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlying the physiological response of potato to US, single-node segments of four-week-old in vitro plantlets were subjected to US at 35 kHz for 20 min. Following mRNA purification, 10 cDNA libraries were assessed by RNA-seq and significantly differentially expressed genes (DEGs) were categorized by gene ontology (GO) or Kyoto Encyclopedia of Genes and Genomes (KEGG) identifiers. The expression intensity of 40,430 genes from a total pool of 45,112 genes were studied. From these, several hundred genes associated with biosynthesis, carbohydrate metabolism and catabolism, cellular protein modification, and response to stress, and expressed mainly in the extracellular region, nucleus, and plasma membrane, were either up- or down-regulated in response to US. This study examines how some processes evolved over time (0 h, 24 h, 48 h, 1 week and 4 weeks) after an abiotic stress (US) was imposed on in vitro potato explants, and provides important clues to the temporal dynamics in enzyme and DEG profiles in response to this stress as the explant becomes established in vitro. Despite this abiotic stress, plantlets survived.

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Solanum tuberosum cultivar:E potato 3

Project description:Transcriptome of Solanum tuberosum 'E potato 3' and Pi04089 transgenic potato

| PRJNA754030 | ENA

Solanum tuberosum cultivar:E potato 3

Project description:Transcriptome of Solanum tuberosum 'E potato 3' and Pi04089 transgenic potato

| PRJNA754010 | ENA

Solanum tuberosum cultivar:E potato 3

Project description:Transcriptome of Solanum tuberosum 'E potato 3' and Pi04089 transgenic potato

| PRJNA754031 | ENA