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RNA-Seq study of blood collected from photosensitive and photorefractory blackheaded buntings exposed to first long day

ABSTRACT: RNA-Seq study of blood collected from photosensitive and photorefractory blackheaded buntings exposed to first long day

PROVIDER: PRJNA558524 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9899550_1.fastq.gz	Fastqsanger.gz
	SRR9899550_2.fastq.gz	Fastqsanger.gz
	SRR9899551_1.fastq.gz	Fastqsanger.gz
	SRR9899551_2.fastq.gz	Fastqsanger.gz
	SRR9899552_1.fastq.gz	Fastqsanger.gz

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RNA-Seq study of blood collected from photosensitive and photorefractory blackheaded buntings exposed to first long day

Project description:We report the mRNA found in blood of photosensitive and photorefractory migratory buntings. The aim of this study was to study the effect of first long day on the mRNA makeup of blood in birds from two different seasonal states.

2019-08-05 | GSE135345 | GEO

The premammillary nucleus (PMM) of the mediobasal hypothalamus: Photosensitive vs. Photorefractory

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Project description:We report the mRNA found in hypothalamus tissue of Spring and Autumn migratory Buntings. The aim of this study was to investigate how the castrations affects mRNA makeup of hypothalamus tissue varies under different seasonal migratory states.

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The change of gene expression during light damage of 661W photosensitive cells

Project description:The changes of iron regulation, pyroptosis, apoptosis and antioxidant genes in 661W photosensitive cells under photodamage were analyzed by next-generation sequencing technology, and specific biomarkers for targeted treatment of retinal photodamage diseases were obtained.

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First description of injury feigning behavior in Godlewski's buntings

Project description:Abstract Nest defense behavior helps ground‐nesting birds improve their breeding success. Among such behaviors, injury feigning behavior (IFB) is one of the better ways to attract predators and protect birds' nests. IFB is generally associated with shorebirds in general and plovers in particular, however, through field observation, it has been found this behavior is also exhibited in bunting species when they facing the risk of predation. We attempt to explore the detailed description of this behavior in buntings and the factors that affect this behavior. Based on video‐recordings of the nest defense behavior by the ground‐nesting bird Godlewski's bunting (Emberiza godlewskii) against nest invaders, we explored the characteristic of IFB and differences in nest defense behavior during their brooding seasons. The results showed that female buntings had a distinct IFB: the wings of buntings incited within 60°–90° of the body, ran and called rapidly, pretending to be injured and unable to fly. The nest stage had significant and extremely significant effects on IFB and movement distance (MD), respectively. And IFB was more likely to occur in brooding (34.62%) than incubation period (16.42%). This indicates that there are significant differences in the IFB of buntings at different breeding stages. Based on video‐recordings of the nest defense behavior by the ground‐nesting bird Godlewskiʼs bunting (Emberiza godlewskii) against nest invaders, we explored the characteristic of IFB and differences in nest defense behavior during their brooding seasons. The results showed that female buntings had a distinct IFB: the wings of buntings incited within 60°–90° of the body, ran and called rapidly, pretending to be injured and unable to fly.

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Transcriptomic Signatures of Postnatal and Adult Intrinsically Photosensitive Ganglion Cells

Project description:Purpose: The goal of this study was to determine a “molecular parts list” for intrinsically photosensitive retinal ganglion cells (ipRGCs), which are rare mammalian photoreceptors essential for non-image-forming vision functions, such as circadian photoentrainment and the pupillary light reflex. Methods: We conducted a thorough unbiased transcriptomic analysis of ipRGCs by purifying GFP-tagged ipRGCs through a combination of FACS and immunoaffinity and comparing this the transcriptional profile of GFP-negative RGCs. We did this in two different mouse lines, marking partially overlapping subsets of ipRGCs. One was a BAC transgenic reporter (Opn4-GFP), which fluorescently labels M1-M3 ipRGCs and the other was a Opn4-Cre;Z/EG reporter system, which labels all ipRGC subtypes, M1-M6 Results: We find dozens of novel genes highly enriched in ipRGCs. We reveal that Rasgrp1 and Tbx20 are selectively expressed in subsets of ipRGCs, though these molecularly defined groups imperfectly match established ipRGC subtypes. We demonstrate that the ipRGCs regulating circadian photoentrainment are unexpectedly diverse at the molecular level. Conclusions: Our study represents the first detailed analysis of ipRGC transcriptomes by RNA-seq. Our findings reveal unexpected complexity in gene expression patterns across mammalian ipRGC subtypes.

2019-08-15 | GSE118780 | GEO

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