Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stress-induced RNA–chromatin interactions promote endothelial dysfunction (Hi-C)

ABSTRACT: Stress-induced RNA–chromatin interactions promote endothelial dysfunction (Hi-C)

PROVIDER: PRJNA558553 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Stress-induced RNA–chromatin interactions promote endothelial dysfunction (Hi-C)

Project description:Chromatin-associated RNA (caRNA) has been proposed as a type of epigenomic modifier. Here, we test whether environmental stress can induce cellular dysfunction through modulating RNA-chromatin interactions. We induce endothelial cell (EC) dysfunction with high glucose and TNFα (H + T), that mimic the common stress in diabetes mellitus. We characterize the H + T-induced changes in gene expression by single cell (sc)RNA-seq, DNA interactions by Hi-C, and RNA-chromatin interactions by iMARGI. H + T induce inter-chromosomal RNA-chromatin interactions, particularly among the super enhancers. To test the causal relationship between H + T-induced RNA-chromatin interactions and the expression of EC dysfunction-related genes, we suppress the LINC00607 RNA. This suppression attenuates the expression of SERPINE1, a critical pro-inflammatory and pro-fibrotic gene. Furthermore, the changes of the co-expression gene network between diabetic and healthy donor-derived ECs corroborate the H + T-induced RNA-chromatin interactions. Taken together, caRNA-mediated dysregulation of gene expression modulates EC dysfunction, a crucial mechanism underlying numerous diseases.

2020-08-17 | GSE135354 | GEO

Stress-induced RNA–chromatin interactions promote endothelial dysfunction

Project description:Stress-induced RNA–chromatin interactions promote endothelial dysfunction

| PRJNA558554 | ENA

Stress-induced RNA–chromatin interactions promote endothelial dysfunction (iMARGI)

Project description:Stress-induced RNA–chromatin interactions promote endothelial dysfunction (iMARGI)

| PRJNA558556 | ENA

Stress-induced RNA–chromatin interactions promote endothelial dysfunction (RNA-seq)

Project description:Stress-induced RNA–chromatin interactions promote endothelial dysfunction (RNA-seq)

| PRJNA657560 | ENA

Stress-induced RNA–chromatin interactions promote endothelial dysfunction (scRNA-seq)

Project description:Stress-induced RNA–chromatin interactions promote endothelial dysfunction (scRNA-seq)