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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RIP-seq]

ABSTRACT: The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RIP-seq]

PROVIDER: PRJNA558575 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9904854.fastq.gz	Fastqsanger.gz
	SRR9904855.fastq.gz	Fastqsanger.gz
	SRR9904856.fastq.gz	Fastqsanger.gz
	SRR9904857.fastq.gz	Fastqsanger.gz
	SRR9904858.fastq.gz	Fastqsanger.gz

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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [ChIP-seq]

Project description:The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [ChIP-seq]

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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RNA-seq]

Project description:The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RNA-seq]

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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [small RNA-seq]

Project description:The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [small RNA-seq]

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ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis

Project description:Heterochromatin de-condensation in companion gametic cells is conserved in both plants and animals. In plants, microspore undergoes asymmetric pollen mitosis (PMI) to produce a vegetative cell (VC) and a generative cell (GC). Subsequently, the GC undergoes pollen mitosis (PMII) to produce two sperm cells (SC). Consistent with heterochromatin de-condensation in the VC, H3K9me2, a heterochromatin mark, is barely detected in VC. However, how H3K9me2 is differentially regulated during pollen mitosis remains unclear. Here, we show that H3K9me2 is gradually evicted from the VC since PMI but remain unchanged in the GC and SC. ARID1, a pollen-specific transcription factor that facilitates PMII, promotes H3K9me2 maintenance in the GC/SC but slows down its eviction in the VC. The genomic targets of ARID1 mostly overlaps with H3K9me2 loci, and ARID1 recruits H3K9 methyltransferase SUVH6. Our results uncover that differential pattern of H3K9me2 between two cell types is regulated by ARID1 during pollen mitosis.

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Cell proliferation suppressor RBR1 interacts with ARID1 to promote pollen mitosis via stabilizing DUO1 in Arabidopsis

Project description:In plants, sperm cell formation involves two rounds of pollen mitoses, in which the microspore initiates the first pollen mitosis (PMI) to produce a vegetative cell and a generative cell, then the generative cell continues the second mitosis (PMII) to produce two sperm cells. DUO1, a R2R3 Myb transcription factor, is activated in the generative cell to promote S-G2/M transition during PMII. Loss-of-function of DUO1 caused a complete arrest of PMII. Despite the importance of DUO1, how DUO1 is regulated is largely unexplored. We previously demonstrated that ARID1, an ARID transcription factor, stimulates DUO1 transcription. Here, we show that cell proliferation suppressor RBR1 interacts with ARID1 to stabilize DUO1. While the C-terminus of RBR1 is dispensable for vegetative growth, it plays a crucial role in reproductive development and facilitates interaction with ARID1. Moreover, DUO1 is a short-lived protein, ARID1 promotes the RBR1-DUO1 interaction, and RBR1 stabilizes DUO1 in a proteasome-dependent manner. Thus, RBR1 promotes DUO1-dependent PMII progression via antagonizing its repressive role in the cell cycle factors CDKA;1 and CYCB1;1. Collectively, we uncover that ARID1 and RBR1 act in concert to regulate DUO1 at both the transcriptional and post-transcriptional levels, balancing cell specification and cell division.

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ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis

Project description:ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis

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Genome-wide maps of SWI/SNF complexes binding in WT and ARID1-less H2.35 cells

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Project description:Single-sperm Sequence Data from Twenty Sperm Donors

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