Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of brown fat in young or old mice in cold exposure

ABSTRACT: Transcriptome analysis of brown fat in young or old mice in cold exposure

PROVIDER: PRJNA558731 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9906420.fastq.gz	Fastqsanger.gz
	SRR9906430.fastq.gz	Fastqsanger.gz
	SRR9906421.fastq.gz	Fastqsanger.gz
	SRR9906412.fastq.gz	Fastqsanger.gz
	SRR9906422.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 19

Similar Datasets

Transcriptome analysis of brown fat in young or old mice in cold exposure

Project description:Purpose: We investigated the transcriptomic change in brown fat of young and old mice (wild type) through high-throughput RNA-sequencing (RNA-Seq) analysis when the mice were exposed to cold room or room temperatur. Methods: We prepared 10 of young (3 months) mice and 9 of old (24 months) mice, and kept them in cold room (4°c) or room temperature (24°c) for 24 hours. Then, we sacrified mice and extracted RNA from brown fat tissue (BAT) for RNA-seq experiment. Results: BAT of Young mice showed increased carbohydrate metabolism and glycolytic flux during cold exposure Conclusions: The thermogenesis function of BAT is accelerated on cold exposure.

2020-07-31 | GSE135391 | GEO

Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C)

Project description:microRNAs levels were measured in brown adipose tissue of male C57Bl6N mice that were kept at RT or during cold exposure (8Â°C) for 24 hrs. Several miRNAs including myomirs were identified to be regulated in response to cold. Brown adipose tissue from 2 groups of 8 weeks old male C57Bl6N mice at room temperature (n=3) or after cold exposure n=3) at 8Â°C for 24 hrs were excised and RNA was extracted using Trizol and the manufactureres recommended procedure. miRNA expression levels were determined using Agilent microRNA expression analysis platform.

2012-11-01 | E-GEOD-41306 | biostudies-arrayexpress

Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C)

Project description:microRNAs levels were measured in brown adipose tissue of male C57Bl6N mice that were kept at RT or during cold exposure (8°C) for 24 hrs. Several miRNAs including myomirs were identified to be regulated in response to cold.

2012-11-01 | GSE41306 | GEO

Short-term cold exposure induces persistent epigenomic memory in brown fat [RNA-Seq]

Project description:Deficiency of the epigenome modulator histone deacetylase 3 (HDAC3) in brown adipose tissue (BAT) impairs the ability of mice to survive in near-freezing temperatures. Here, we report that short-term exposure to mild cold temperature (STEMCT: 15°C for 24 hours) averted lethal hypothermia of mice lacking HDAC3 in BAT (HDAC3 BAT KO) exposed to 4°C. STEMCT restored the induction of the thermogenic coactivator PGC-1a along with UCP1 at 22°C, which is greatly impaired in HDAC3-deficient BAT, and deletion of either UCP1 or PGC-1a prevented the protective effect of STEMCT. Remarkably, the protection of HDAC3 BAT KO mice from cold intolerance following STEMCT lasted for up to 7 days. Transcriptional activator C/EBPb was induced by short-term cold exposure in mouse and human BAT and, uniquely, remained high for 7 days following STEMCT. Furthermore, analysis of C/EBPb activity revealed increased binding following STEMCT at genes, including the enhancers/promotors of UCP1 and PGC-1a. These results reveal the existence of a cold-adaptive epigenomic memory mediated by C/EBPb that is persistent and HDAC3-independent.

2024-07-05 | GSE244385 | GEO

Short-term cold exposure induces persistent epigenomic memory in brown fat [GRO-Seq]

2024-07-05 | GSE244384 | GEO

Short-term cold exposure induces persistent epigenomic memory in brown fat [ChIP-Seq

2024-07-05 | GSE244383 | GEO

Short-term cold exposure induces persistent epigenomic memory in brown fat [ATAC-Seq]

2024-07-05 | GSE244382 | GEO

Cold-induction of Afadin in Brown Fat supports its thermogenic capacity

Project description:Transcriptomic analysis of brown fat from fat-specific Afadin depleted mice either at thermoneutrality or after 6 houts of cold exposure (5 degree celsius)

2021-05-12 | GSE147392 | GEO

Transcriptome profiling of brown adipose tissue during cold exposure

Project description:We applied a deep-sequencing based method – digital gene expression profiling (DGEP), to investigate gene expression in interscapular brown adipose tissue (iBAT), inguinal white adipose tissue (iWAT) and epididymal white adipose tissue (eWAT) in acute cold exposure

2014-12-19 | GSE63031 | GEO

Transcriptome profiling of brown adipose tissue during cold exposure

2014-12-19 | E-GEOD-63031 | biostudies-arrayexpress