Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Difference of GATA4 and beta-catenin pulldown chromatin immunoprecipitated DNA in HePG2i cells line with and without DOX induced GATA4 expression

ABSTRACT: Difference of GATA4 and beta-catenin pulldown chromatin immunoprecipitated DNA in HePG2i cells line with and without DOX induced GATA4 expression

PROVIDER: PRJNA559839 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9951330.fastq.gz	Fastqsanger.gz
	SRR9951331.fastq.gz	Fastqsanger.gz
	SRR9951332.fastq.gz	Fastqsanger.gz
	SRR9951327.fastq.gz	Fastqsanger.gz
	SRR9951328.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Difference of GATA4 and beta-catenin pulldown chromatin immunoprecipitated DNA in HePG2i cells line with and without DOX induced GATA4 expression

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of HePG2i cell line with and without DOX induced GATA4 expression by obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA.

2019-08-13 | GSE135714 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of HePG2i cell line with and without DOX induced GATA4 expression Transcriptomes

Project description:HePG2i cell line mRNA profiles of DOX induced GATA4 expression and non-DOX induced were generated by deep sequencing, in triplicate, using Illumina HiSeq 10000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

2019-08-09 | GSE135579 | GEO

ChIP-seq of GATA4 in mouse skeletal muscle myoblasts

Project description:GATA4 occupancy on the mouse genome of satellite cell-derived primary myoblasts. Proliferating myoblasts cultured in growth medium were immunoprecipitated with anti-GATA4 antibody or control IgG. Precipitated genomic DNAs were subjected to next generation sequencing.

2015-04-10 | GSE67703 | GEO

ChIP-seq of GATA4 in mouse skeletal muscle myoblasts

2015-04-10 | E-GEOD-67703 | biostudies-arrayexpress

RNA pulldown target circGLS2

Project description:RNA pulldown target circGLS2

| PRJNA786500 | ENA

MicroRNA expression signatures for gata4/5/6 knocking down in zebrafish

Project description:Previous works showed that gata4/5/6 lie at the hierarchical apex of the regulatory network of the hemangioblast formation and primitive myelopoiesis in zebrafish. To explore the roles of miRNAs in zebrafish primitive myelopoiesis, we employed deep sequencing to analyze the difference of miRNA expression profiles between gata4/5/6 knockdown embryos (gata5/6 morphants) and control embryos.

2021-08-14 | GSE118850 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of HePG2i cell line with and without DOX induced GATA4 expression Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of HePG2i cell line with and without DOX induced GATA4 expression Transcriptomes

| PRJNA559361 | ENA

Cardiac transcription factors in HL-1 cells: gene expression and genome binding profiling

Project description:[1] Gene expression profiling in Gata4, Mef2a knockdown in HL-1 cells. HL-1 cells infected with adenovirus expressing either control siRNA or Gata4, and Gata4 & Mef2a siRNA. [2] Genome-wide maps of cardiac transcription factors binding region in HL-1 cells. We performed bio-ChIP-seq using streptavidin beads immunoprecipitation of biotinylated 5 cardiac transcription factors (fbio) and P300 antibody ChIP-seq. We used a dox-inducible dual adenovirus system to express biotinylated Core Cardiac TFs in HL1. One adenovirus expressed the dox-activated reverse tet activator protein (rtTA) and the E. coli biotinylating enzyme BirA from the cardiac specific rat troponin T promoter. The second virus expressed a Core Cardiac TF fused to a C-terminal flag-bio epitope tag, where bio is the 15 amino acid substrate for BirA. This in vivo biotinylation approach permits pulldown of different factors to be performed under identical, stringent conditions, and circumvents limitations posed by available antibodies. We titrated adenovirus and dox doses to express GATA4flbio and MEF2Aflbio at near endogenous levels. The same conditions were then used to express SRFflbio, TBX5flbio, and NKX2-5flbio. Reference: 12. de Boer E, Rodriguez P, Bonte E, Krijgsveld J, Katsantoni E et al. (2003) Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A 100: 7480-7485. [1] Gene expression profiling: Identify differentially expressed genes after siRNA Gata4 or Gata4_Mef2a double knockdown in HL-1 cells [2] Genome occupancy profiling: Identify cardiac active enhancers by identified 5 cardiac transcription factors and P300 peaks.

2011-03-02 | E-GEOD-21529 | biostudies-arrayexpress

FoxP3 Pulldown-seq (Nucleosomal DNA)

Project description:FoxP3 Pulldown-seq (Nucleosomal DNA)

| PRJNA1018996 | ENA

miR-146a Pulldown-sequencing data

Project description:miR-146a Pulldown-sequencing data

| PRJNA675568 | ENA