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Aeorestes semotus

ABSTRACT: One-way ticket to paradise: new methods for analysis of genotype sequence data reveals the origin and evolutionary separation of Hawaiian hoary bat populations

PROVIDER: PRJNA559902 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9961970_1.fastq.gz	Fastqsanger.gz
	SRR9961970_2.fastq.gz	Fastqsanger.gz
	SRR9961980_1.fastq.gz	Fastqsanger.gz
	SRR9961980_2.fastq.gz	Fastqsanger.gz
	SRR9961971_1.fastq.gz	Fastqsanger.gz

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Aeorestes semotus

Project description:Genetic diversity, structure and effective population size of an endangered, endemic hoary bat, across the Hawaiian Islands

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Project description:Brown adipose tissue (BAT) evolved in mammals as a natural defence system against hypothermia and obesity. While existence of BAT in adult humans has been recently appreciated, its cellular origin and molecular identity remain elusive due in large to high cellular heterogeneity within adipose tissues. Here we isolated clonal adipocytes from adult human BAT as well as WAT (control) and critically analyzed their transcriptome to identify bona fide BAT markers and its new functions.

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ilEucCana

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RNAseq for CDK7 brown adipose tissue specific knockout mice under room temperature and 6 hrs cold exposure.

Project description:Purpose: The goal of this study are to compare global gene expression profile in brown adipose tissue in Cdk7 bKO and WT control mice at both RT and after 6 hours cold exposure Methods: Interscapular BAT pads were taken from 12-week-old CDK7 bKO and control littermates either at room temperature or after 6 hours of cold exposure with food. Mice were sacrificed at zeitgeber time 9. Total RNA was extracted with TRI Reagent (Sigma). Two RNA samples from two mice with same genotype and condition were pool together (each 250 ng) to constitute 500 ng of total RNA from biological triplicates (three WT-RT, three CDK7 bKO-RT, three WT-cold, and three CDK7 bKO-cold) for sequencing. Results: Reads were also aligned to the Mus musculus.GRCm38.86 transcriptome using STAR (v. 2.5.2b, Dobin et al. 2013) and the estimation of the isoforms abundance was computed using RSEM (v. 1.2.31, Li and Dewey 2011). Conclusions: Differentially expressed genes (DEGs) were identified between genotype and treatment.

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SIRT3 controls brown fat thermogenesis by deacetylation regulation of pathways upstream of UCP1  

Project description:Objective: Brown adipose tissue (BAT) is important for thermoregulation in many mammals. Uncoupling protein 1 (UCP1) is the critical regulator of thermogenesis in BAT. Here we aimed to investigate the deacetylation control of BAT and to investigate a possible functional connection between UCP1 and sirtuin 3 (SIRT3), the master mitochondrial deacetylase.   Methods: We carried out physiological, molecular and proteomic analyses of BAT from wild-type and Sirt3KO mice when BAT is activated. Mice were either cold exposed for 2 days or were injected with the β3-adrenergic agonist, CL316,243 (1mg/kg; i.p.). Mutagenesis studies were conducted in a cellular model to assess the impact of acetyaltion lysine sites on UCP1 function. Cardiac punctures were collected for Proteomic analysis of Acylcarnitines. Isolated mitochondria were used for functional analysis of OXPHOS.   Results: Our findings showed that SIRT3 absence in mice resulted in impaired BAT lipid use, whole body thermoregulation, and respiration in BAT mitochondria, without affecting UCP1 expression. Acetylome profiling of BAT mitochondria revealed that SIRT3 regulates acetylation status of many BAT mitochondrial proteins including UCP1 and crucial upstream proteins. Mutagenesis work in cells suggested that UCP1 activity was independent of direct SIRT3-regulated lysine acetylation. However, SIRT3 impacted BAT mitochondrial activities of acylcarnitine metabolism and specific electron transport chain complexes, CI and CII.  

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