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Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

ABSTRACT: Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

PROVIDER: PRJNA560773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9994493_1.fastq.gz	Fastqsanger.gz
	SRR9994493_2.fastq.gz	Fastqsanger.gz
	SRR9994494_1.fastq.gz	Fastqsanger.gz
	SRR9994494_2.fastq.gz	Fastqsanger.gz
	SRR9994495_1.fastq.gz	Fastqsanger.gz

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Phenotype and function of ex vivo expanded liver progenitor-like cells through liver parenchymal and non-parenchymal reversion

Project description:The cellular source for hepatocyte transplantation and liver regeneration in chronically hepatic diseases is a fundamental topic in liver biology. A successful therapeutic approach based on a benificial stem/progenitor cells would involve replacing damaged cells or restoring homeostasis to the areas that underlie the fibrotic response. This study aims to isolate and culture hepatocyte and non-parenchymal cells derived liver progenitor-like cells (HepLPCs and NPC-LPCs) to identify a beneficial cell sources for cell-based therapy against chronic liver injury. Two types of lineage tracing models including tdTomato mice with AAV8-TBG-Cre and AlbCreERT/R26GFP were purified for hepatocytes and non-parenchymal cells respectively, and cultured for transition to progenitor-like cells (LPCs) in TEM. The differences between them were clarified by RNA sequencing, suggesting that HepLPCs retaining basal levels of hepatic function and transcription factors (TFs), including Foxa3. Organoids and 3D hepatic spheroids culturing systems were used for further analysis of bile duct and hepatic functioning diversity. When transplanting in mice with CCL4 treatment, HepLPCs displayed the characteristics of TFs regulatory reactivation and physiological balance restoration, resulting in a significant improvement in liver fibrosis compared with NPC-LPCs, which are crucial for liver regeneration in the treatment of patients with a broad range of hepatic fibrosis.

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Phenotype and function of ex vivo expanded liver progenitor-like cells through biliary epithelial cells reversion

Project description:Phenotype and function of ex vivo expanded liver progenitor-like cells through biliary epithelial cells reversion

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Phenotype and function of ex vivo expanded liver progenitor-like cells through biliary epithelial cells reversion

Project description:The current study combined genetic lineage tracing with in vitro small-molecule-based reprogramming to define liver progenitor cells (LPCs) derived from hepatic parenchymal and non-parenchymal cells (NPCs). tdTom+ hepatocytes were isolated from ROSA26tdTomato mice following AAV8-Tbg-Cre-mediated recombination, ALB/GFP-EpCAM- NPCs were isolated from AlbCreERT/R26GFP mice and EpCAM+ biliary epithelial cells (BECs) from wild-type intrahepatic bile ducts. A cocktail of small molecules was used to convert the isolated cells into LPCs.

2024-07-24 | GSE238160 | GEO

TAK1 deficient liver parenchymal cells with Rip3 double knockout TAK1LPC

Project description:Genomic copy number profiling of TAK1 deficient liver parenchymal cells with Rip3 double knockout Sample vs. Reference (pool of 4 normal liver tissues)

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Single-cell RNA sequencing of liver parenchymal cells from murine model of endotoxemia

Project description:Liver has a crucial role in the regulation of immune defense in systemic infections. During endotoxemia, the liver transits from an immune-tolerant towards an immune-active state and forms the first line of defense against invading microorganisms. the role of liver parenchymal cells in endotoxemia remains unintelligible. To characterize the liver parenchymal cells in endotoxemia liver, we performed single-cell RNA sequencing of liver parenchymal cells from healthy C57BL/6J mice and murine model of endotoxemia at early or late stage. The single-cell RNA-seq analyses revealed the heterogeneity of liver parenchymal cells in endotoxemia liver.

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TAK1 deficient liver parenchymal cells with Rip3 double knockout TAK1LPC

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RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

Project description:NK cells were isolated from the perpheral blood of healthy donors (n=6) and expanded ex vivo in the presence of feeder cells and IL-2. RNA from fresh and expanded cells was isolated, sequenced, and gene expression of the cell populations were compared.

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Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages

Project description:Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages

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Smart-seq2 single-cell RNA sequencing of mouse liver non-parenchymal cells

Project description:Smart-seq2 scRNA-seq of liver non-parenchymal cells from 3 high-fat diet and 3 control mice.

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Gene expression signature of ex vivo expanded NK cells for 14 days

Project description:we performed RNA sequencing to employ an unbiased approach to identify the altered gene expression enhancing the function of ex vivo expanded pNK cells for 14 days, and transcriptionally compared day 0 and day 14 pNK cells.

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