Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression data from HEK293T cells stably expressing human TRAF7 wildtype or G536S mutant isoforms

ABSTRACT: Gene expression data from HEK293T cells stably expressing human TRAF7 wildtype or G536S mutant isoforms

PROVIDER: PRJNA560939 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression data from HEK293T cells stably expressing human TRAF7 wildtype or G536S mutant isoforms

Project description:The TRAF7 gene is recurrently mutated in meningiomas, adenomatoid tumors of the genital tract, well-differentiated papillary mesotheliomas, and intraneural perineuriomas. However, the mechanisms by which these recurrent missense mutations contribute to tumorigenesis has not yet been described. To elucidate the transcriptional differences between HEK293T cells overexpressing wildtype vs mutant TRAF7, we used Affymetrix microarrays to identify the gene transcripts that are upregulated and downregulated depedent on TRAF7 status.

2021-06-01 | GSE136012 | GEO

ChIP-seq analyis of SVG-p12 human fetal glial cells with exogenous expression of wildtype SOX10 or schwannoma-derived mutant isoforms

Project description:To investigate the chromatin binding patterns of wildtype SOX10 versus schwannoma-derived mutant isoforms, we established SVG-p12 cell lines stably expressing either FLAG epitope tagged wildtype SOX10 or two different mutant isoforms of SOX10. We then performed chromatin immunoprecipitation-DNA sequencing on these SVG-p12 cell lines stably transduced with wildtype or mutant SOX10 isoforms using an anti-FLAG epitope antibody.

2023-07-13 | GSE234394 | GEO

Transcriptomic analyis of SVG-p12 human fetal glial cells with overexpression of wildtype SOX10 or schwannoma-derived mutant isoforms

Project description:To investigate the gene expression changes caused by wildtype versus schwannoma-derived mutations in SOX10, we established SVG-p12 cell lines stably expressing either empty pCDF1 vector, wildtype SOX10, or two different mutant isoforms of SOX10. We then performed gene expression profiling analysis using RNA-seq for these SVG-p12 cell lines stably transduced with wildtype or mutant SOX10 isoforms.

2023-07-13 | GSE218097 | GEO

Next-generation sequencing facilitates transcriptomes quantitative analysis of stably expressing RfxCas13d HEK293T cells transfected with target genes and corresponding crRNAs

Project description:To determine what effect the collateral activity of RfxCas13d has on cells, a HEK293T cell line stably expressing RfxCas13d (HEK293T-RfxCas13d) was constructed and then transfected with plasmids encoding target gene and corresponding crRNAs. After 24 hours of transfection, cells were collected and extracted for total RNA. Then, we performed RNA-seq to compare the differentially expressed genes between cells transfected with targeting crRNAs and non-targeting crRNA.

2022-02-10 | GSE193667 | GEO

Transcriptome analysis of melanoma cells stably expressing BMP6

Project description:Transcriptome analysis of melanoma cells stably expressing BMP6

| PRJNA690945 | ENA

Interactome of overexpressed BAG3 in HEK293T cells

Project description:HEK293T cells stably expressing N-terminally HA-tagged BAG3 were employed to screen for novel BAG3 interacting proteins.

2019-10-10 | PXD014473 | Pride

Differences in gene expression patterns in mouse embryonic fibroblast Flp-In-3T3 cell lines stably expressing Pax6 or Pax6(5a).

Project description:Novel target genes for the two transcription factor isoforms Pax6 and Pax6(5a) were identified by generating mouse embryonic fibroblast cell lines stably expressing either Pax6 or Pax6(5a), and comparing their gene expression pattern with the original mouse embryonic fibroblast cell line.

2012-03-22 | GSE31402 | GEO

ChIP-sequencing of Human FOXN2 and RFX1 in HEK293T Cells

Project description:We have performed ChIP-sequencing analysis on human FOXN2 and RFX1 target sequences in human embryonic kidney HEK293T cells stably expressing Streptavidin-S-FLAG (SFB) triple-tagged proteins. The NGS sequencing were performed on Illumina MiSeq desktop sequencer.

2015-01-01 | E-MTAB-3120 | biostudies-arrayexpress

Gene expression data from MCF7 cells stably expressing COX7RP

Project description:Gene expression data from MCF7 cells stably expressing COX7RP

| PRJNA512291 | ENA

Expression (Gene) array from AMl12-Zfp125- stably expressing cells

Project description:Expression (Gene) array from AMl12-Zfp125- stably expressing cells

| PRJNA419404 | ENA