Project description:Rheumatoid arthritis (RA) is a chronic, inflammatory joint disease of unknown etiology and pronounced inter-patient heterogeneity. To characterize RA at the molecular level and to uncover key pathomechanisms, we performed whole-genome gene expression analyses. Synovial tissues from rheumatoid arthritis patients were compared to those from osteoarthritis patients and to normal donors. Keywords: disease state analysis

2004-11-04 | GSE1919 | GEO

Comprehensive Profiling of Rheumatoid Arthritis Antibody Repertoire

Project description:8 controls and 26 RA samples were profiled using NimbleTherapeutics high density peptide array. Array consists of >4.6M peptides, includes citrullination and homocitrullination against the entire human proteome represented as overlapping 16mer peptides.

2019-10-21 | GSE136052 | GEO

Comprehensive Epigenetic Landscape of Rheumatoid Arthritis Fibroblast-like Synoviocytes [WGBS]

Project description:Comprehensive Epigenetic Landscape of Rheumatoid Arthritis Fibroblast-like Synoviocytes [WGBS]

| PRJNA448633 | ENA

Antigen-specific Fab profiling achieves molecular resolution of human autoantibody repertoires in rheumatoid arthritis

Project description:The presence of autoantibodies is a defining feature of many autoimmune diseases. The number of unique autoantibody clones is conceivably limited by immune tolerance mechanisms, but unknown due to limitations of the currently applied technologies. Here, we introduce an autoantigen-specific liquid chromatography-mass spectrometry-based IgG1 Fab profiling approach using the anti-citrullinated protein antibody (ACPA) repertoire in rheumatoid arthritis (RA) as an example. We show that each patient harbors a unique and diverse ACPA IgG1 repertoire dominated by only a few antibody clones. In contrast to the total plasma IgG1 antibody repertoire, the ACPA IgG1 sub-repertoire is characterized by an expansion of antibodies that harbor one, two or even more Fab glycans, and different glycovariants of the same clone can be detected. Together, our data indicate that the autoantibody response in a prominent human autoimmune disease is complex, unique to each patient and dominated by a relatively low number of clones.

2023-10-26 | MSV000093196 | MassIVE

Differential DNA methylation in Rheumatoid arthritis

Project description:Genome-wide DNA methylation level was studied to determine whether Rheumatoid arthritis patients (cases) has methylation differences comparing to normal controls in PBLs. We used Illumina HumanMethylation450 BeadChip array to determine the genome-wide DNA methylation difference in PBLs from Rheumatoid arthritis patients (cases) and normal controls Bisulphite converted DNA from the Rheumatoid arthritis patients (cases) and normal controls were hybridized to the Illumina Illumina HumanMethylation450 BeadChip arrays

2013-01-21 | E-GEOD-42861 | biostudies-arrayexpress

single-cell transcriptomics in patients with anti-citrullinated peptide antibody-positive early rheumatoid arthritis

Project description:Despite known differences in immunologic features between anti-citrullinated peptide antibody (ACPA)-positive (ACPA+) and ACPA-negative (ACPA-) early rheumatoid arthritis (eRA) patients, our understanding is still incomplete. To address this, we performed single-cell RNA sequencing of CD45+ cells from peripheral blood samples of drug-naïve ACPA+ and ACPA- eRA patients and compared distribution and functional characteristics of cell subsets based on ACPA presence.

2025-01-13 | GSE260796 | GEO

Rheumatoid arthritis: aortic biopsies

Project description:Rheumatoid arthritis: aortic biopsies

| PRJNA432552 | ENA