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ABSTRACT: microRNA in American cockroach

PROVIDER: PRJNA561668 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10015080.fastq.gz	Fastqsanger.gz
	SRR10015081.fastq.gz	Fastqsanger.gz
	SRR10015082.fastq.gz	Fastqsanger.gz
	SRR10015083.fastq.gz	Fastqsanger.gz

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Project description:microRNA in American cockroach

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Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach

Project description:As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. Using large-scale peptidomic analysis of peptide extracts from 4 different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) and a potential (novel) neuropeptides were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of 2 conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide PaOGS36577. Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach.

2020-11-12 | PXD021660 | Pride

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Project description:In this study, the fresh oothecae used for proteomics analysis were obtained from American cockroach. Proteomics and PRM analysis were used for the identification of the oothecal proteins including the structural proteins and key enzymes.

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Project description:Raw Genome Sequences of American cockroach (Periplaneta americana)

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Project description:Single Cell Genomes from the Hindgut of the American Cockroach (Periplaneta americana)

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MicroRNA profiles discriminate among colon cancer metastasis

Project description:The aim of our study was to identify a microRNA signature for metastatic CRC that could predict and differentiate metastatic target organ localization. Normal and cancer tissues of three different groups of CRC patients were analyzed. RNA microarray and TaqMan Array analysis were performed on 66 italian patients with or without lymph nodes and/or liver recurrences. Data obtained with the two assays, were analyzed separately and then intersected to identify a primary CRC metastatic signature. Five differentially expressed microRNAs (hsa-miR-21, -103, -93, -31 and -566) were validated by qRT-PCR on a second group of 16 american metastatic patients. In situ hybridization was performed on the 16 american patients as well as on three distinct commercial tissues microarray (TMA), containing normal adjacent colon, the primary adenocarcinoma, normal and metastatic lymph nodes and liver. Hsa-microRNA-31,-21,-93, and-103 upregulation together with hsa-miR-566 downregulation defined the CRC metastatic signature, while in situ hybridization data identified a lymphonodal invasion profile.

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