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CytoScan HD array data from Human mammary epithelian cells after Cyclin E overexpression

ABSTRACT: CytoScan HD array data from Human mammary epithelian cells after Cyclin E overexpression

PROVIDER: PRJNA562026 | ENA |

REPOSITORIES: ENA

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CytoScan HD array data from Human mammary epithelian cells after Cyclin E overexpression

Project description:Cyclin E, a key cell cycle regulatory protein, has been linked to oncogenesis when dysregulated. We have previously shown that overexpression of cyclin E causes replication stress, leading to failure to complete replication at specific chromosomal loci during S phase of the cell cycle. This, in turn promotes chromosomal damage during anaphase. Here we show that non-transformed human mammary epithelial cell clones that survive such aberrant mitoses, have a specific and reproducible pattern of chromosomal Copy Number Alterations (CNAs) that we have characterized and termed the cyclin E CNA signature. Using a number of computational approaches, we show that this signature resembles one specific CNA pattern enriched in differentiated epithelial-like tumors of the breast and ovary. We further show that this CNA signature translates into expected altered patterns of transcription. In addition, we show that 2 out of 12 clones analyzed were capable of anchorage-independent growth, suggesting acquisition of sufficient oncogenic transformation properties. Analysis of the CNA profile of these clones provides a potential mechanism for cyclin E-mediated oncogenesis.

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two amniotic fluid sample performed by affymetrix cytoscan HD

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