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C. elegans PPM-2 regulates small RNA function by stabilizing Argonaute proteins

ABSTRACT: C. elegans PPM-2 regulates small RNA function by stabilizing Argonaute proteins

PROVIDER: PRJNA562557 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10046090.fastq.gz	Fastqsanger.gz
	SRR10046091.fastq.gz	Fastqsanger.gz
	SRR10046092.fastq.gz	Fastqsanger.gz
	SRR10046093.fastq.gz	Fastqsanger.gz

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C. elegans PPM-2 regulates small RNA function by stabilizing Argonaute proteins

Project description:Argonaute proteins associate with small non-coding RNAs such as microRNAs, piRNAs and siRNAs to regulate gene expression required for cell proliferation and differentiation. Thse Argonaute proteins are the major components of effector complexes of non-coding RNA pathways. In C. elegans, we identified PPM-2, a Mg2+/Mn2+-dependent serine/threonine phosphatase, as a new regulator of the small RNA pathways in C. elegans. We demonstrate that PPM-2 is involved in the regulation of the let-7 microRNA family but also in the regulation of nuclear RNAi as well as it contributes to the expression of piRNAs. The loss of ppm-2 affects the expression level of Type 1 piRNAs and the stability of ALG-1, PRG-1 and NRDE-3 Argonaute proteins by sending them to the proteasomal degradation pathway. Altogether, our findings suggest that PPM-2 is regulating the function of small non-coding RNA pathways by preventing Argonaute proteins to be degraded via the proteasomal pathway.

2021-08-01 | GSE136462 | GEO

A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [Argonaute IP]

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Argonaute proteins couple chromatin silencing to alternative splicing

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A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing

Project description:Germline Argonautes direct transcriptome surveillance within peri-nuclear membraneless organelles called nuage. In C. elegans, a family of Vasa-related Germ Line Helicase (GLH) proteins localize in, and promote the formation of nuage called P granules. Previous studies have implicated GLH proteins in inherited silencing but direct roles in amplification of small RNAs, or in target mRNA or Argonatue binding have not been identified. Here we show that GLH proteins compete with each other to control Argonaute pathway specificity, bind directly to Argonaute-target mRNAs and act to promote the amplification of small RNAs required for transgenerational inheritance. We show that the ATPase cycle of GLH-1 regulates its direct binding to the Argonaute WAGO-1 which engages amplified small RNAs. Our findings support a dynamic and direct role for GLH proteins in inherited silencing beyond their role as structural components of nuage.

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Modeling neurodevelopmental disorder-associated human AGO1 mutations in C. elegans Argonaute alg-1. [ribosome profiling]

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