Ontology highlight
ABSTRACT:
PROVIDER: PRJNA563034 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The bacterium, Sinorhizobium meliloti, interacts symbiotically with leguminous plants such as Medicago truncatula to form nitrogen-fixing root nodules. During symbiosis, plant and bacterial cells differentiate in a coordinated manner, resulting in specialized plant cells that contain nitrogen-fixing bacteroids. Medicago nodules are organized in structurally distinct tissue zones, representing different stages of bacterial and plant differentiation. We used laser-capture microdissection (LCM) to analyze bacterial and plant gene expression in four root nodule regions. In parallel, we analyzed gene expression in nodules formed by wild type bacteria on six plant mutants with nitrogen fixation deficiencies (dnf). We found that bacteroid metabolism is drastically remodeled during bacteroid differentiation. Many processes required for bacterial growth are down-regulated in the nitrogen fixation zone. The overall transcriptional changes are similar to those occurring during nutrient limitation by the stringent response. We also observed differential expression of bacterial genes involved in nitrogen fixation, cell envelope homeostasis, cell division, stress response and polyamine biosynthesis at distinct stages of nodule development. In M. truncatula we observed the differential regulation of several host processes that may trigger bacteroid differentiation and control bacterial infection. We analyzed plant and bacterial gene expression simultaneously, which allowed us to correlate processes in both organisms.
2015-06-01 | GSE53705 | GEO
Project description:Gene expression in mature, nitrogen-fixing root nodules was compared to gene expression in uninoculated roots.
2005-06-30 | E-MEXP-237 | biostudies-arrayexpress
Project description:Prosopis cineraria microbiomes - 16S, NifH, shotgun metagenome and metatranscriptome reads
| PRJNA881730 | ENA
Project description:The transformation of Sinorhizobium bacteria into nitrogen-fixing bacteroids located in the root-nodules of the Medicago plants is controlled by specific peptides released by the plants. In our study root nodules, and differently purified bacteroid mixtures were studied. Detailed description about sample preparation and data interpretation can be found in "Identification of Nodule-Specific Cysteine-Rich Plant Peptides in Endosymbiotic Bacteria" by H. Durgo et al., Proteomics (2015). CID spectra supporting all NCR identifications can be viewed using MS-Viewer on the public Protein Prospector website (prospector.ucsf.edu), search key: h7swi0wcik.
2015-02-17 | MSV000079037 | MassIVE
Project description:De novo assembly and characterization of leaf transcriptome of Prosopis cineraria
| PRJNA371524 | ENA
Project description:Metabolomics and transcriptomics of Bradyrhizobium diazoefficiens-induced root nodules Bradyrhizobium diazoefficiens is a nitrogen-fixing endosymbiont, which can grow inside root-nodule cells of the agriculturally important soybean and other host plants. Our previous studies described B. diazoefficiens host-specific global expression changes occurring during legume infection at the transcript and protein level. In order to further characterize nodule metabolism, we here determine by flow injection -time of flight mass spectrometry analysis the metabolome of i) nodules and roots from four different B. diazoefficiens host plants, ii) soybean nodules harvested at different time points during nodule development, and iii) soybean nodules infected by two strains mutated in key genes for nitrogen fixation, respectively. Ribose (soybean), tartaric acid (mungbean), hydroxybutanoyloxybutanoate (siratro) and catechol (cowpea) were among the metabolites found to be specifically elevated in one of the respective host plants. While the level of C4-dicarboxylic acids decreased during soybean nodule development, we observed an accumulation of trehalose-phosphate at 21 days post infection (dpi). Moreover, nodules from non-nitrogen-fixing bacteroids (nifA and nifH mutants) showed specific metabolic alterations; these were also supported by transcriptomics data that was generated for the two mutant strains and were helpful to separate for some examples the respective bacterial and plant contributions to the metabolic profile. The alterations included signs of nitrogen limitation in both mutants, and an increased level of a phytoalexin in nodules induced by the nifA mutant, suggesting that the tissue of these nodules exhibits defense and stress reactions.
2016-11-28 | GSE79811 | GEO