Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Best Practices for the Analysis of Oxford Nanopore Direct RNA Sequencing Data, m5C

PROVIDER: PRJNA563591 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13570753_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Best Practices for the Analysis of Oxford Nanopore Direct RNA Sequencing Data

| PRJNA548268 | ENA

Project description:Best Practices for the Analysis of Oxford Nanopore Direct RNA Sequencing Data, pU

| PRJNA549001 | ENA

Project description:Best Practices for the Analysis of Oxford Nanopore Direct RNA Sequencing Data, UNM-S

| PRJNA575545 | ENA

Project description:Best Practices for the Analysis of Oxford Nanopore Direct RNA Sequencing Data, Varying Stoichometry pU Curlcake

| PRJNA695584 | ENA

Short and long read sequencing of human mammary epithelial MCF10a-Snail-ER cells after Epithelial-to-Mesenchymal Transition initiation

Project description:We cultured MCF10a-Snail-ER cells and induced EMT initiation with tamoxifen. A matched sequencing of their PolyA RNA was performed, using Illumina and direct RNA Oxford Nanopore sequencing technologies. Both generated datasets supported the development of hybrid bioinformatics tools.

2021-09-09 | GSE126638 | GEO

Nanopore RNA-seq of polyA+ RNA from HAP1 and HEK293 cell lines

Project description:Sequencing was performed to assess the ability of Nanopore direct cDNA and native RNA sequencing to characterise human transcriptomes. Total RNA was extracted from either HAP1 or HEK293 cells, and the polyA+ fraction isolated using oligodT dynabeads. Libraries were prepared using Oxford Nanopore Technologies (ONT) kits according to manufacturers instructions. Samples were then sequenced on ONT R9.4 flow cells to generate fast5 raw reads in the ONT MinKNOW software. Fast5 reads were then base-called using the ONT Albacore software to generate Fastq reads.

2019-03-14 | E-MTAB-7778 | biostudies-arrayexpress

Examining RNA Transcript Changes in Stem Cells Undergoing Differentiation, Using Short and Long Reads Sequencing Technologies [Illumina short reads]

Project description:We explored changes at gene-level or transcript-level in embryonic stem cells, before and after in vitro differentiation with retinoic acid. RNA was sequenced both via Illumina short reads, and with Oxford Nanopore Technology with cDNA and direct RNA sequencing.

2022-09-04 | GSE156370 | GEO

Examining RNA Transcript Changes in Stem Cells Undergoing Differentiation, Using Short and Long Reads Sequencing Technologies [Nanopore long reads]

2022-09-04 | GSE156369 | GEO

Direct RNA sequencing enables single-nucleotide m6A detection in endogenous transcript isoforms

Project description:We report the direct RNA sequencing of HEK293 and a primary human mammary epithelial cell (HMEC) line using Oxford Nanopore based sequencing. Using this data, we built an algorithm to detect m6A modifications within the DRACH motif context. Evaluation of m6A sites was carried out with HEK METTL3 knockdown and HMEC ALKBH5 over expression cell lines.

2020-01-14 | GSE132971 | GEO

Oxford nanopore sequencing data from the Arabidopsis thaliana accessions Ler-0, Cvi-0 and Tanz-1

Project description:Oxford nanopore sequencing data from the Arabidopsis thaliana accessions Ler-0, Cvi-0 and Tanz-1

2022-08-06 | E-MTAB-12009 | biostudies-arrayexpress