Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of jejunal and ileal mucosa from postnatal growth retardation and healthy pigs

ABSTRACT: Real-time quantitative PCR analysis of jejunal and ileal mucosa from postnatal growth retardation and healthy pigs

PROVIDER: PRJNA563649 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Real-time quantitative PCR analysis of jejunal and ileal mucosa from postnatal growth retardation and healthy pigs

Project description:The purpose of this study was to investigate the relative mRNA expression related to hormone, antioxidant capacity and immune responses in jejunal and ileal mucosa of healthy and postnatal growth retardation pigs. At 42 d of age, after overnight fasting, six postnatal growth retardation pigs and six healthy pigs were pair-matched by litter were selected for sampling. Samples of the jejunal and ileal mucosa were scraped and immediately snap-frozen in liquid nitrogen and stored at −80°C for RNA extraction. We used Roche LightCycler ® 480 Instrument PCR assay panel to quantitate gene expression of hormone, antioxidant capacity and immune responses relevant genes from jejunal and ileal mucosa.

2019-09-04 | GSE136777 | GEO

murine jejunal and ileal epithelial tissue

Project description:murine jejunal and ileal epithelial tissue

| PRJNA595855 | ENA

Open Array Custom Real-time quantitative PCR analysis of mouse ileal mucosa

Project description:Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the ileal and colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays.

2019-10-01 | GSE121612 | GEO

Undernutrition disrupts jejunal and ileal microbiota and epithelial tissue homeostasis in a pregnant sheep model

Project description:In the current study, five late gestation multiparous ewes were restricted to a 30% feeding level to create a model of malnutrition, while five other ewes were fed normally as controls. All ewes were sacrificed and jejunal and ileal samples were collected for transcriptome sequencing to study metabolic changes.

2024-11-07 | GSE236643 | GEO

Open Array Mouse Inflammation Real-time quantitative PCR analysis of mouse ileal mucosa

Project description:Intrarectally DNBS-treated groups, to provoke colitis, were treated orally with the commensal CEC (CEC) or probiotic Nissle 1917 (Nissle) E. coli strains. Healthy control group was treated intrarectally and orally with PBS (PP). Sick control group was treated intrarectally with DNBS and orally with PBS (DP). Mice were sacrificed 24 days post first DNBS injection and the ileal gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays Mouse Inflammation Panel.

2019-10-01 | GSE121613 | GEO

murine jejunal and ileal epithelial tissue

Project description:Transcriptional profiling of mouse jejunal epithelia comparing Tcrbd-/-, DAOwt/wt mouse and Tcrbd-/-, DAOG181R/G181R Intestinal microbiota produce D-amino acids, which are bacteria-specific metabolites, for regulation of bacterial cell wall integrity. Host intestine releases D-amino acid oxidase (DAO) to degrade bacterial D-amino acids, which shapes gut microbial community. However, it is not clarified whether bacterial D-amino acids affect host s immunity. In the present study, we compared mRNA expression in ileal epithelial tissue of DAO null mice with that of control mice in the absence of both T cell receptor beta and delta (Tcrb/d). Transcriptome analysis revealed up-regulation of inflammatory cytokines such as TNFa, IL1b, and IFNg in the epithelial tissue. Up-regulation of such cytokines could promote survival of na ve B cells and increase IgA-producing plasma cells, which in turn results in enhancement of IgA production. These results indicate that DAO controls intestinal immune responses through regulation of na ve B cells and differentiation of mature B cells.

2020-08-31 | GSE142070 | GEO

The duodenal, ileal, jejunal and caecal microbiota in chickens (16s)

Project description:The duodenal, ileal, jejunal and caecal microbiota in chickens (16s)

| PRJEB33615 | ENA

Jejunal transcriptome profiles of pigs divergent in feed efficiency

Project description:in vivo microarray study of transcriptional changes of jejunal scratchings (mucosa) obtained from pigs divergent in feed efficiency.

2018-12-21 | GSE109332 | GEO

Open Array Custom Real-time quantitative PCR analysis of mouse ileal mucosa

Project description:Open Array Custom Real-time quantitative PCR analysis of mouse ileal mucosa

| PRJNA497871 | ENA

Open Array Mouse Inflammation Real-time quantitative PCR analysis of mouse ileal mucosa

Project description:Open Array Mouse Inflammation Real-time quantitative PCR analysis of mouse ileal mucosa

| PRJNA497874 | ENA