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RNA Sequencing of C elegans wild type (N2) grown on 20 vs 5µg/ml cholesterol

ABSTRACT: RNA Sequencing of C elegans wild type (N2) grown on 20 vs 5µg/ml cholesterol

PROVIDER: PRJNA563909 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10067886.fastq.gz	Fastqsanger.gz
	SRR10067887.fastq.gz	Fastqsanger.gz
	SRR10067888.fastq.gz	Fastqsanger.gz
	SRR10067889.fastq.gz	Fastqsanger.gz
	SRR10067890.fastq.gz	Fastqsanger.gz

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RNA Sequencing of C elegans wild type (N2) grown on 20 vs 5µg/ml cholesterol

Project description:Purpose: Cholesterol is an essential nutrient for diverse biological processes in all organisms. However, the role of cholesterol in immune function remains understudied. Hence the goal is to obtain cholesterol-mediated immune genes Methods: Hermaphrodite C. elegans (var. Bristol) wild type (N2) were grown on 20 along side with control, which is 5µg/ml cholesterol and harvested at L4 stage. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analyses of the transcriptomic data show that cholesterol-mediated immune gene that could enhance the fight against infectious diseases Conclusions: This study showed a novel role for cholesterol in the enhancement of the innate

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