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Alteration of genome folding via engineered transposon insertion [H3K27Ac ChIP-seq]

ABSTRACT: Alteration of genome folding via engineered transposon insertion [H3K27Ac ChIP-seq]

PROVIDER: PRJNA565258 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10108613.fastq.gz	Fastqsanger.gz
	SRR10108614.fastq.gz	Fastqsanger.gz
	SRR10108615.fastq.gz	Fastqsanger.gz
	SRR10108616.fastq.gz	Fastqsanger.gz
	SRR12041755.fastq.gz	Fastqsanger.gz

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Project description:Mammalian chromosomes are partitioned into contact domains that can be conserved as functional units in evolution. Disruptions of domains can result from perturbed CTCF, cohesin, or chromosomal rearrangements. However, to what extent domains can be created de novo has not been explored in depth. Here, using a gain-of-function approach leveraging genome editing and Hi-C, we examined whether, and how, a putative boundary element can function to organize de novo domains in the context of multiple ectopic insertion sites. We subsequently dissected the distinct roles of the CTCF binding site and the transcription start site within the insertion element in changing genome folding.

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Project description:Alteration of genome folding via engineered transposon insertion [RNA-seq]

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