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The acute transcriptome response of the midbrain/diencephalon to injury in the adult mummichog (Fundulus heteroclitus)

ABSTRACT: The acute transcriptome response of the midbrain/diencephalon to injury in the adult mummichog (Fundulus heteroclitus)

PROVIDER: PRJNA565537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10120344.fastq.gz	Fastqsanger.gz
	SRR10120345.fastq.gz	Fastqsanger.gz
	SRR10120346.fastq.gz	Fastqsanger.gz
	SRR10120347.fastq.gz	Fastqsanger.gz
	SRR10120348.fastq.gz	Fastqsanger.gz

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The acute transcriptome response of the midbrain/diencephalon to injury in the adult mummichog (Fundulus heteroclitus)

Project description:Adult fish produce new cells throughout their central nervous system during the course of their lives. Furthermore, they maintain a tremendous capacity to repair damaged neural tissue. Much of the focus on understanding brain repair and regeneration in fish has been directed at regions of the brainstem and forebrain; however, the mesencephalon (midbrain) and diencephalon have received comparatively little attention. We sought to characterize differential gene expression in the midbrain/diencephalon in response to injury in the adult fish using RNA-seq. Using the mummichog (Fundulus heteroclitus), we administered a mechanical lesion traversing the midbrain optic tectum, tegmentum and underlying hypothalamus of the diencephalon and examined differential gene expression at an acute recovery time of 1hr post-injury. Comparisons of whole transcriptomes derived from isolated RNA of intact and injured midbrain/diencephalic tissue identified over 400 differentially expressed genes with the vast majority being upregulated. Using qPCR, we validated the upregulation of three differentially expressed genes, pim-2-like, syndecan-4-like, and cd83. Based on genes both familiar and novel regarding the adult brain response to injury, these data provide an extensive molecular profile giving insight into a range of cellular processes putatively involved in the injury response of a brain regenerative-capable vertebrate.

2020-06-03 | GSE137451 | GEO

Fundulus heteroclitus heteroclitus

Project description:Fundulus heteroclitus heteroclitus RADseq reads

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Adult male Fundulus heteroclitus exposed to Cr(VI)

Project description:Cr(VI) is a common bioavailable toxic metal that can cause oxidative stress, DNA adducts, and perturb normal gene expression. Changes in gene expression are useful biomarkers of toxicant exposure that provide information about the health of an organism, its ability to adapt to its environment, and indicate potential toxicant-specific effects. Therefore, we developed a toxicology array to the estuarine sentinel species Fundulus heteroclitus, or mummichog. Adults males were exposed to Cr(VI) for 7-days at 0, 1.5 (NOEC), or 3 mg/L (LOEC). Livers were excised and RNA isolated. Adults are used in the laboratory experiments so that we can compare laboratory studies to fish caught at chromium-contaminated field sites. Cr(VI) altered the expression of 12 genes in adult liver, including hepatic growth factor activator, heart fatty acid binding protein, and complement component C3-2. Keywords: dose response

2006-03-31 | GSE3480 | GEO

Fundulus heteroclitus

Project description:Effects of 17α-ethinylestradiol (EE2) on molecular signaling cascades in mummichog (Fundulus heteroclitus)

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Juvenile Fundulus heteroclitus exposed to Cr(VI)

Project description:Cr(VI) is a common bioavailable toxic metal that can cause oxidative stress, DNA adducts, and perturb normal gene expression. Changes in gene expression are useful biomarkers of toxicant exposure that provide information about the health of an organism, its ability to adapt to its environment, and indicate potential toxicant-specific effects. Therefore, we developed a toxicology array to the estuarine sentinel species Fundulus heteroclitus, or mummichog. Juvenile mummichog were exposed to potassium dichromate for thirty days at concentrations from 0 to 24 mg/L of Cr(VI), and growth was measured to determine the NOEC (1.5 mg/L or 0.0288 mM) and LOEC (3 mg/L or 0.0577 mM). Body burdens from Cr(VI) exposed fish demonstrated a dose dependent increase and were inversely correlated to body weight. Cr(VI)-exposed juvenile mummichog differentially expressed greater than 20 genes in a dose-dependent manner, including hepatic glucose transporter 2, liver fatty acid binding protein, ATPase synthase 8, type II keratin, TBT binding protein, and complement component C3-2. Many of these genes are involved in energy metabolism or growth, which is consistent with the reduced growth caused by Cr(VI). Keywords: dose response

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Project description:Fundulus heteroclitus Genome sequencing

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Technical Analysis of Fundulus heteroclitus cDNA Microarrays

Project description:This SuperSeries is composed of the following subset Series: GSE12858: Technical Analysis of Fundulus heteroclitus cDNA Microarrays, experiment A GSE12898: Technical Analysis of Fundulus heteroclitus cDNA Microarrays, experiment B Refer to individual Series

2010-05-15 | E-GEOD-13687 | biostudies-arrayexpress

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