Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Alkali sediment metagenome

ABSTRACT: alkali sediment metagenome Raw sequence reads

PROVIDER: PRJNA565614 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10123223.fastq.gz	Fastqsanger.gz
	SRR10123224.fastq.gz	Fastqsanger.gz
	SRR10123225.fastq.gz	Fastqsanger.gz
	SRR10123226.fastq.gz	Fastqsanger.gz
	SRR10123227.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 17

Similar Datasets

alkali sediment metagenome

Project description:alkali sediment metagenome Raw sequence reads

| PRJNA433101 | ENA

alkali sediment metagenome

Project description:alkali sediment metagenome Raw sequence reads gujun

| PRJNA565666 | ENA

alkali sediment metagenome

Project description:alkali sediment metagenome Metagenomic assembly

| PRJNA414565 | ENA

alkali sediment metagenome

Project description:alkali sediment metagenome Targeted loci environmental

| PRJNA323418 | ENA

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

Project description:Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc kgc tg-3’), extended as amplicon fusion primers with respective primer A or B adapters, key sequence and multiplex identifiers (MID) as recommended by 454/Roche. Amplicons were purified and pooled as specified by the manufacturer. Emulsion PCR (emPCR), purification of DNA-enriched beads and sequencing run were performed following protocols and using a 2nd generation pyrosequencer (454 GS FLX Titanium, Roche) as recommended by the developer. Quality filtering of the pyrosequencing reads was performed using the automatic amplicon pipeline of the GS Run Processor (Roche), with a slight modification concerning the valley filter (vfScanAllFlows false instead of TiOnly) to extract the sequences. Demultiplexed raw reads were furhter trimmed for quality and lenght (>250 bp).

2012-11-11 | GSE25345 | GEO

Saline alkali soil

Project description:Saline alkali soil Raw sequence reads

| PRJNA542419 | ENA

Saline-Alkali Soil

Project description:Saline-Alkali Soil Raw sequence reads

| PRJNA949358 | ENA

Saline alkali soil

Project description:Saline alkali soil Raw sequence reads

| PRJNA542413 | ENA

saline alkali soil

Project description:saline alkali soil Raw sequence reads