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An alternative transcriptome shapes cell fate transitions in yeast (TES-seq)

ABSTRACT: An alternative transcriptome shapes cell fate transitions in yeast (TES-seq)

PROVIDER: PRJNA566361 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10145100.fastq.gz	Fastqsanger.gz
	SRR10145101.fastq.gz	Fastqsanger.gz
	SRR10145102.fastq.gz	Fastqsanger.gz
	SRR10145103.fastq.gz	Fastqsanger.gz
	SRR10145104.fastq.gz	Fastqsanger.gz

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An alternative transcriptome shapes cell fate transitions in yeast (TES-seq)

Project description:Alternative mRNA isoforms and long noncoding RNAs (lncRNA) make up a large fraction of the transcriptome and play key functions in cell-fate programming. These transcripts often initiate upstream of coding gene promoters from alternative transcription start sites (TSS) where they can regulate gene expression in cis through transcription-coupled chromatin alterations. How, when and where transcription of alternative cis-acting RNAs regulates local gene expression remains poorly understood. Here, we use a high-resolution quantitative approach to study alternative TSS and transcript end site (TES) usage during three different cell fate transitions in yeast: entry into gametogenesis, commitment to meiotic divisions and return to vegetative growth. We propose that an alternative transcriptome of mRNA isoforms and lncRNAs shapes local gene expression during cell fate transitions. Hence, changes in the types and proportions of different RNAs transcribed at a locus are important inputs for gene expression at distinct stages of development.

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Project description:An alternative transcriptome shapes cell fate transitions in yeast (TIF-seq)

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