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Genetic networks regulating structure and function of the maize shoot apical meristem

ABSTRACT: Genetic networks regulating structure and function of the maize shoot apical meristem

PROVIDER: PRJNA566382 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10145220.fastq.gz	Fastqsanger.gz
	SRR10145221.fastq.gz	Fastqsanger.gz
	SRR10145222.fastq.gz	Fastqsanger.gz
	SRR10145223.fastq.gz	Fastqsanger.gz
	SRR10145224.fastq.gz	Fastqsanger.gz

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Genetic networks regulating structure and function of the maize shoot apical meristem

Project description:The shoot apical meristem (SAM) of plants, a specialized stem cell niche at the growing shoot tip, integrates developmental and environmental signals to direct the initiation and patterning of new organs such as leaves. Its activity throughout the plant's lifetime is tightly controlled. To gain insight into gene regulatory networks behind stem cell maintenance and organogenesis, we generated a high-resolution gene expression atlas of distinct domains and cell types within the vegetative shoot apex of 14 day-old maize B73 seedlings using laser microdissection and RNA deep sequencing. Data for 9 domains was previously released under SRA: SRP101301. This release adds data for biological duplicate samples collected for the SAM central zone, and the adaxial and abaxial sides of leaf primordia.

2019-10-02 | GSE137715 | GEO

Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

Project description:gnp07_regeneome_transdifferenciation - microdissection - Study of the moleculars mecanism during transdifferenciation of Root ApicalMeristem to Shoot Apical Meristem - middle of growth permits to induce transdifferenciation of root apical meristem to shoot apical meristem

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Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

2012-05-31 | E-GEOD-30259 | biostudies-arrayexpress

Transdifferentiation from root to shoot apical meristem

Project description:au10-15_cineroots - transdifferentiation - Study of the molecular mechanism during transdifferenciation from root apical meristem to shoot apical meristem - culture in middle with different hormons, permits transdifferenciation from root to shoot tissues.

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Transdifferentiation from root to shoot apical meristem

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Chromatin states in the Arabidopsis shoot apical meristem

Project description:We investigated the chromatin modifications H3K4me3 and H3K27me3 in the A. thaliana shoot apical meristem using INTACT reporter lines. Samples were collected in two biological replications.

2017-05-21 | E-MTAB-4684 | biostudies-arrayexpress

Gene Expression between two cell layers in maize shoot apical meristem

Project description:All above ground organs of higher plants are ultimately derived from shoot apical meristems (SAMs). The SAM exhibits distinctive structural organization, and monocot SAMs such as maize are comprised of two cell layers, a single cell layered tunica (L1) and a corpus (L2). Although recent research has revealed roles of these cell layers in the SAM, intra- and inter-cell-layer signaling networks involved in organ development remain largely unknown except for a few differentially expressed genes. Here, we used Illumnia technology to conduct RNA-seq of L1 and L2 cell layers in maize B73 maize shoot apical meristem. Single sequencing library was constructed for L1 and L2 cell layer. Each library was sequenced using 2 lanes on a Solexa flow cell. Processed data file 'ZmB73_4a.53_filtered_genes.fasta' and its README file are linked below as supplementary files. The fasta file contains the gene model ID and corresponding sequence generated from maize genome project. This fasta file was used for the following samples: GSM418173, GSM418174, GSM420173, GSM420174, GSM422828, GSM422829.

2013-01-28 | E-GEOD-16868 | biostudies-arrayexpress

Chromatin states in the Arabidopsis shoot apical meristem

Project description:Chromatin states in the Arabidopsis shoot apical meristem

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Proteome of rice shoot apical meristem

Project description:DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we found that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also found that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

2020-08-14 | PXD020608 | Pride

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