Project description:To elucidate role of IRE1 in Candida albicans, genome wide expressional profile change was compared by microarray analysis between wild type (SC5314) and IRE1 mutant (ire1 DX) cells after successful RNA extraction. Expression of six genes (HWP1, ECE1, SOD3, AMS1, YWP1, SIT1 and FET34) was validated by qPCR and observed to have similar expression pattern when compared to their relative expression in microaaray data.
Project description:Goal: We employed RNA-seq to identify targets of regulation of the Candida albicans transcription regulator CUP9. The cup9 deletion mutant strain displays increased fitness in a mouse model of oropharyngeal candidiasis.
Project description:To elucidate the impact of IFU5 in Candida albicans, genome wide transcription profiling was performed in ifu5?/? mutant strain. Wild type and mutant cells were grown for 5 hours and RNA extracted from these cultures, followed by microarray profiling. Expression of six genes (EFG1, ALS3, SOD3, BMT4, COX2, NAD1) was validated by qPCR.