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Linker-mediated PCR for global integration site analysis in splenic human CD4-positive T cells of HIV1-GFP-infected humanized mice

ABSTRACT: Linker-mediated PCR for global integration site analysis in splenic human CD4-positive T cells of HIV1-GFP-infected humanized mice

PROVIDER: PRJNA574039 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10181142_1.fastq.gz	Fastqsanger.gz
	SRR10181142_2.fastq.gz	Fastqsanger.gz
	SRR10181143_1.fastq.gz	Fastqsanger.gz
	SRR10181143_2.fastq.gz	Fastqsanger.gz
	SRR10181144_1.fastq.gz	Fastqsanger.gz

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Linker-mediated PCR for global integration site analysis in splenic human CD4-positive T cells of HIV1-GFP-infected humanized mice

Project description:To comprehensively compare the HIV-1 integration sites between viral producing cells (GFP+ cells) and non-producing cells (GFP- cells), linker-mediated PCR is performed by using the samples obtained from HIV1-GFP-infected humanized mice. Additionally, to reactivate latently infected cells, viral non-producing cells (GFP- cells) were stimulated with PMA and ionomycin ex vivo. Then, the viral integration sites in potential latently infected cells (GFP-_GFP+ cells) and the cells with dead/inactivated proviruses (GFP-_GFP- cells) were analyzed by linker-mediated PCR.

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