Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Uncultured mixed community

ABSTRACT: Uncultured mixed community Raw sequence reads

PROVIDER: PRJNA574652 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10203902_1.fastq.gz	Fastqsanger.gz
	SRR10203902_2.fastq.gz	Fastqsanger.gz
	SRR10204344_1.fastq.gz	Fastqsanger.gz
	SRR10204344_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Uncultured mixed community

Project description:Uncultured mixed community Raw sequence reads

| PRJNA497444 | ENA

Mixed silage

Project description:Mixed silage Raw sequence reads

| PRJNA1170237 | ENA

Micribe mixed

Project description:Micribe mixed Raw sequence reads

| PRJNA542201 | ENA

Vinyl Chloride Degradation by Mixed Microbial Community KB1

Project description:A shotgun metagenome microarray was created and used to investigate gene transcription during vinyl chloride (VC) dechlorination by a microbial enrichment culture called KB1. The array was constructed by spotting genomic fragments amplified from short-insert libraries of KB1 metagenomic DNA. Subsequently, the microarrays were interrogated with RNA extracted from KB1 during VC dechlorination (VC+methanol), and in the absence of VC (methanol-only). The most differentially expressed spots, and spots with the highest intensities, were then chosen to be sequenced. Sequencing revealed that Dehalococcoides (Dhc) genes involved in transcription, translation and energy generation were up-regulated during VC degradation. Furthermore, the results indicated that the reductive dehalogenase homologous (RDH) gene KB1rdhA14 is the only RDH gene up-regulated upon VC degradation, and that multiple RDH genes were more highly transcribed in the absence of VC. Numerous hypothetical genes from Dehalococcoides were also more highly transcribed in methanol only treatments and indicate that many uncharacterized proteins are involved in cell maintenance in the absence of chlorinated substrates. Spots with genes from Spirochaetes, Chloroflexi, Geobacter, Methanogens and phage organisms were differentially expressed and sequencing provided information from these uncultivated organisms that can be used to design primers for more targeted studies. This array format is powerful, as it does not require a priori sequence knowledge. This study provides the first report of such arrays being used to investigate transcription in a mixed community, and shows that this array format can be used to screen metagenomic libraries for functionally important genes.

2010-01-27 | GSE20041 | GEO

uncultured microorganism

Project description:uncultured Raw sequence reads

| PRJNA352883 | ENA

mixed culture bacterium

Project description:mixed culture bacterium Raw sequence reads

| PRJNA869916 | ENA

mixed culture metagenome

Project description:mixed culture metagenome Raw sequence reads

| PRJNA326444 | ENA

mixed culture metagenome

Project description:mixed culture metagenome Raw sequence reads