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Exome Sequencing of Ackr4-deficient mice in generation 12 (backcrossing to C57BL/6)

ABSTRACT: Exome Sequencing of Ackr4-deficient mice in generation 12 (backcrossing to C57BL/6)

PROVIDER: PRJNA574668 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10197437_1.fastq.gz	Fastqsanger.gz
	SRR10197437_2.fastq.gz	Fastqsanger.gz
	SRR10197438_1.fastq.gz	Fastqsanger.gz
	SRR10197438_2.fastq.gz	Fastqsanger.gz
	SRR10197439_1.fastq.gz	Fastqsanger.gz

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Exome Sequencing of Ackr4-deficient mice in generation 12 (backcrossing to C57BL/6)

Project description:Purpose: The goal of this study was to compare exome sequences of Ackr4-deficient mice with C57BL/6 wildtype sequences to understand phenotypic differences which can not be explained by Ackr4-deficiency. Methods: Tail DNA of one wild-type (WT, C57BL/6) and atypical chemokine receptor 4 knockout (Ackr4−/−) mice was sequenced by exome sequencing using an Illumina NovaSeq 6000 sequencing system (2x 100bp). After demultiplexing (Illumina bcl2fastq 2.19) and adapter trimming (Skewer, 0.2.2), the trimmed reads were aligned with to the murine genome (mm10) with Burrows-Wheeler Aligner (BWA-mem 0.7.2-cegat). Reads aligned to multiple sides with the same mapping score were discarded. Further, duplicated reads were removed with SAMtool 0.1.18. Genetic variants were identified with Verscan 2.4.2-cegat and variants with a frequency of ≥ 2% were annotated with SnpEff (version 4.2 with GRCm38.75). Results: 100,000 to 140,000 million sequence reads per mouse were mapped to the mouse genome (build mm10). Each mouse had approx. 20,000 variants (insertions, deletions, SNPs) compared to the reference genome. In Ackr4-/- mice, 3,367 variants were present in both mice analysed, yet not detected in the C57BL/6 mouse used as control. 1781 of these 'shared' variants were present on the chromosome with the targeted Ackr4 allele (Chr9) and predominantly in close proximity to the Ackr4 locus, indicating that these variants originate from the 129-genetic background which was used for targeted mutagenesis during the generation of these mice. Conclusions: Our study shows that Ackr4-/- mice in generation F12 after backcrossing to the C57BL/6 background are still congenic for 129-derived DNA around the targeted Ackr4 locus, indicating that passenger mutations can influence the phenotype of these mice.

2019-12-21 | GSE138124 | GEO

Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants

Project description:Mycothiol (MSH) and ergothioneine (ERG) are thiols able to compensate for each other to protect mycobacteria such as Mycobacterium smegmatis against oxidative stress. Gamma-glutamylcysteine (GGC), another thiol and an intermediate in ERG biosynthesis has detoxification abilities. Five enzymes are involved in ERG biosynthesis, namely EgtA, EgtB, EgtC, EgtD and EgtE. The role of these enzymes in the production of ERG had been unclear. On the other hand, the enzyme MshA is known to be essential for MSH biosynthesis. In this manuscript, we describe the raw data of the generation and characterization of Mycobacterium tuberculosis (M.tb) mutants harbouring a deletion of the gene coding for each of these enzymes, and the raw data of the phenotypic characterization of the obtained thiol-deficient M.tb mutants. Through a high throughput screening approach, compounds not previously used in a tuberculosis (TB) regimen were identified. Raw data depicting the effect of these compounds on the generated thiol-deficient M.tb mutants, on the production of reactive oxygen species (ROS) and thiols in the M.tb wild type strain are described in this study.

2018-07-05 | MTBLS661 | MetaboLights

Panicum virgatum cultivar:SAP-12-12

Project description:Panicum virgatum exome capture - SAP-12-12

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Mouse spleen LC-MSMS from C57BL/6J WT, CD38-deficient and B6.C-H2bm12/KHEg (bm12) mice in cGVHD lupus-like model

Project description:Proteins from post-nuclear cell lysates from C57BL/6J WT, CD38-deficient and B6.C-H2bm12/KHEg (bm12) recipient mice at 2 weeks after the adoptive transfer of co-isogenic spleen cells were identified using a proteomic approach. The relative abundances of the proteins identified were calculated using emPAIs scores.

2021-10-11 | PXD026947 | Pride

ATAC-seq analysis of murine thymic iNKT subsets from C57BL/6 mice and Hivep3-deficient mice

Project description:ATAC-seq analysis of murine thymic iNKT subsets from two independent C57BL/6 mice and two independent Hivep3-deficient mice

2020-10-31 | GSE160518 | GEO

Clinical exome sequencing of cells freshly isolated from 12 human colorectal carcinoma patients (tumor endothelial cells, normal colon endothelial cells, PBMCs, n=12 each) in comparison to microdisected tumor DNA (n=11) from corresponding FFPE-tissue blocks

Project description:Clinical exome sequencing of cells freshly isolated from 12 human colorectal carcinoma patients (tumor endothelial cells, normal colon endothelial cells, PBMCs, each n=12) in comparison to DNA isolated from microdissected tumor cells (n=11) from corresponding FFPE-tissue blocks

2023-05-01 | E-MTAB-10521 | biostudies-arrayexpress

Transcriptome of CD8+T-cells from C57BL/6 mice in induced tumor-like conditions along with IL-2 and IL-12.

Project description:To study differentially expressed genes via RNA seq analysis of CD8+T-cells from C57BL/6 mice splenocytes treated with the four different conditions: IL-2, IL-2 80%TCM, IL-12 and IL-12 80%TCM. (TCM = Tumor Conditioned Media)

2021-11-23 | GSE189170 | GEO

Next Generation Sequencing of Wild-Type C57BL/6J Mouse Cardiac Polyadenylated RNA

Project description:Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in several publications involving mouse hearts, dating from 2010, and wish to share both high-quality raw sequencing data and data processed to quantitate mRNA abundances from wild-type mice, male and female, at a variety of ages (see our FVB/NJ data submission). These data will provide a resource for investigators using microarrays to understand the concentration of transcripts of interest relative to other cardiac RNAs, and will permit deeper interpretation of previous microarray studies. 4 cardiac polyA+-RNA profiles of 12 week-old C57BL/6J wild type (WT) mice were generated on Illumina HiSeq 2000 instruments.

2014-03-12 | E-GEOD-55788 | biostudies-arrayexpress

Gene expression analysis of in vitro activated B cells from C57BL/6 and Ackr4-deficient mice

Project description:To further understand differential phenotypes between C57BL/6 and Ackr4-deficient B cells, we have employed whole genome microarray expression profiling as a discovery platform to identify genes which are differentially expressed in the two genotypes. Murine B cells from spleens of C57BL/6 and Ackr4-deficient mice were purified by negative magnetic activated cell sorting (MACS) and activated in vitro with 10 µg/mL polyclonal anti-IgM and10 µg/mL anti-CD40 (clone FGK4.5) antibodies for three days in culture (96well plate, 37°C, 5%CO2). On day three, living B cells were sorted and RNA was extracted.

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Profiling of exome mutations associated with progression of HBV-related hepatocellular carcinoma

Project description:We profiled the mutations and gene expressions of early and advanced hepatocellular carcinoma (HCC) related with Hepatitis B-viral infection. Integrative analysis was performed with whole-exome sequencing and gene expression profiles of the 12 cases of early and advanced HCCs and paired non-tumoral adjacent liver tissues.

2014-10-19 | GSE62441 | GEO

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