Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analyzing the transcriptomic response of MCF7 breast cancer cells to NR1H4 agonist GW4064

ABSTRACT: Analyzing the transcriptomic response of MCF7 breast cancer cells to NR1H4 agonist GW4064

PROVIDER: PRJNA574916 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Analyzing the transcriptomic response of MCF7 breast cancer cells to NR1H4 agonist GW4064

Project description:Computational analysis identified NR1H4 as an active nuclear receptor in a xenograft model of breast cancer. Since there is evidence that NR1H4 agonist, GW4064, reduces MCF7 cell proliferation, here, we wanted understand the transcriptional changes induced by activating this nuclear receptor. We measured changes in gene expression due to GW4064-treatment and applied gene set enrichment analysis to further understand the perturbed molecular pathways.

2019-10-01 | GSE138138 | GEO

Gene expression profiles of MCF7 with with the following NR ligands: estradiol/E2 (ERα agonist), AM580 (RARA agonist), CD437 (RARG agonist), ATRA (RARA/RARG agonist), GW0742 (PPARD agonist), R5020 (PGR agonist), and dexamethasone (GR/NR3C1 agonist).

2013-01-01 | GSE42619 | GEO

Gene expression profiles of MCF7 with with the following NR ligands: estradiol/E2 (ERÎ± agonist), AM580 (RARA agonist), CD437 (RARG agonist), ATRA (RARA/RARG agonist), GW0742 (PPARD agonist), R5020 (PGR agonist), and dexamethasone (GR/NR3C1 agonist).

Project description:Altered gene expression patterns in human diseases reflect perturbations in the transcriptional networks that regulate cellular state. In breast cancer, Nuclear Receptors (NRs) play a prominent role in governing gene expression. NRs have prognostic utility and are therapeutically important targets. In order to understand the function of nuclear receptors in breast cancer, we treated MCF7 cells with 6 nuclear receptor agonist, and performed expression arrrays with three biological replicates each. MCF-7 cells were cultured for 48 hours in medium with 10% charcoal-stripped FBS, and then were treated with 6 diffferent NR ligand (E2, AM580, CD437, ATRA, R5020, DEX) separately for 24h before collection and processing for microarrays. Three biological replicates were collected and processed for both control and treatment. with the 100nM GW0742 (PPARD specific ligand) and 100nM ATRA (RARs ligand), then collected after 24h for microarray processing. Controls were non-treated MCF7 cells. Both treatments and controls have three biological replicates.

2013-01-01 | E-GEOD-42619 | biostudies-arrayexpress

Insulin stimulated MCF7 breast cancer cells

Project description:Proteome profile of MCF7 breast cancer cells stimulated with insulin and analyzed by nano-LC-ESI-MS/MS.

2016-11-30 | PXD004051 | Pride

FOXK2 geneknockown induces transcriptomic changes in breast cancer cell line MCF7

Project description:To investigate the effect of FOXK2 knockdown on transcriptomic profiling in breast cancer and delineate the role of FOXK2 in breast cancer growth. We knockdowned the gene exprssion of FOXK2 in MCF7 cells and performed gene expression profiling analysis using data obtained from RNA-seq.

2023-09-11 | GSE230623 | GEO

Transcriptomic profiles of MCF7-derived tamoxifen resistant cell lines

Project description:We report mRNA profiles of human breast cancer cell lines, MCF7 parental, and MCF7-derived tamoxifen resistant cell lines MCF7-TR1 and MCF7-TR2.

2023-10-25 | GSE241654 | GEO

Identification of ZNF217 interactors in MCF7 breast cancer cell line

Project description:Identification of TRPS1 interactors in MCF7 breast cancer cell line.

2018-07-17 | PXD010312 | Pride

Gene expression from MCF7 breast cancer cells

Project description:Gene expression from MCF7 breast cancer cells at different times of TNFa incubation:pcs2 and 14-3-3 transduced cells Keywords: Expression Profiling by array We analyzed 2 arrays from each condition:MCF7-control tnf 0, MCF7-control tnf 20min, MCF-control tnf 90min, MCF7-14-3-3 tnf 0, MCF7-14-3-3 tnf 20min, MCF7-14-3-3 tnf 90min

2012-04-12 | E-GEOD-37139 | biostudies-arrayexpress

HAX1-interactome in breast cancer cell line (MCF7)

Project description:The MCF7 cell line represents a typical epithelial cell line and corresponds to luminal A breast cancer (estrogen-responsive). Overexpression of HAX1 was demonstrated in MCF7 cell line as well as in breast cancer samples, suggesting a role of HAX1 in breast cancer progression. HAX1 is a 32-kDa protein of unknown structure, involved in the regulation of apoptosis, cell migration and calcium homeostasis. It was also shown to bind mRNA. Scarcity of structural elements and the presence of a disordered region, inferred from HAX1 sequence, suggests that HAX1 is intrinsically disordered, and may have many protein-protein interactions. So far about 40 different proteins were characterized as HAX1 protein partners. In the present work, applying immunoaffinity chromatography coupled with mass spectrometry, we identified new candidates for HAX1 binding partners in breast cancer cells. Newly identified proteins may be divided into three, partially overlapping groups: cytoskeleton-associated proteins, GTP-ase associated proteins and RNA-binding proteins. These results imply that HAX1 has more protein partners than hitherto described. Subsequent analysis of these interactions may shed some light into molecular mechanisms of HAX1 functions.

2019-11-14 | PXD007888 | Pride

Gene expression from MCF7 breast cancer cells

Project description:Gene expression from MCF7 breast cancer cells at different times of TNFa incubation:pcs2 and 14-3-3 transduced cells Keywords: Expression Profiling by array

2012-04-13 | GSE37139 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data