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Organism Dual RNA-Seq Reveals the Control on both Pathogen and Host through BarA/UvrY of Vibrio parahaemolyticus for Successful Infection

ABSTRACT: Organism Dual RNA-Seq Reveals the Control on both Pathogen and Host through BarA/UvrY of Vibrio parahaemolyticus for Successful Infection

PROVIDER: PRJNA575028 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10207230_1.fastq.gz	Fastqsanger.gz
	SRR10207230_2.fastq.gz	Fastqsanger.gz
	SRR10207231_1.fastq.gz	Fastqsanger.gz
	SRR10207231_2.fastq.gz	Fastqsanger.gz
	SRR10207232_1.fastq.gz	Fastqsanger.gz

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Organism Dual RNA-Seq Reveals the Control on both Pathogen and Host through BarA/UvrY of Vibrio parahaemolyticus for Successful Infection

Project description:Elucidation of host-pathogen interaction is essential for developing effective strategies to combat bacterial infection. Dual RNA-Seq using cultured cells or tissues/organs as the pathogen host has emerged as a novel strategy to understand the response concurrently from both pathogen and host at cellular level. However, bacterial infection mostly causes systematic responses from the host at organism level where the interplay is urgently to be understood but inevitably being neglected by the current practice. Here, we developed an approach that simultaneously monitor the genome-wide infection-linked transcriptional alterations in both pathogenic Vibrio parahaemolyticus and the infection host nematode Caenorhabditis elegans. Besides the dynamic alterations in both transcriptomes of C. elegans and V. parahaemolyticus during infection, we identify a two-component system, BarA/UvrY, that are essential for host colonization. BarA/UvrY not only controls the virulence factors in V. parahaemolyticus including Type III and Type VI secretion systems, but also represses the MAP kinase mediated signaling cascades to attenuate innate immune responses. Thus, our study exemplifies the use of dual RNA-Seq at organism level to uncover previously unrecognized interplay between host and pathogen.

2020-09-10 | GSE138199 | GEO

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Vibrio parahaemolyticus gene expression affected by 0.04% bile

Project description:Vibrio parahaemolyticus is a Gram-negative marine bacterium. Strain RIMD 2210633, the wild type strain of the organism, causes acute gastroenteritis in humans. Human intestinal factor bile often affects the global gene regulation in some species of enteropathogenic bacteria. To determine the genes in V. parahaemolyticus that respond to bile, we investigated the differences in the transcriptomes of the wild type strain and the vtrA-null strain grown in Luria-Bertani medium cultivated with or without 0.04% crude bile. The vtrA gene encodes the previously identified T3SS2 regulator. Our goal is to demonstrate bile regulon controlled by VtrA in V. parahaemolyticus.

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Successful Clinical Response in Pneumonia Therapy (SCRIPT)

Project description:Successful Clinical Response in Pneumonia Therapy (SCRIPT)

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Successful Clinical Response in Pneumonia Therapy (SCRIPT)

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Project description:Human and mouse organism part comparison data set in which organism parts are exactly matched, or are component parts of matching organism parts, between the two species. Data obtained from E-AFMX-4 and E-AFMX-5.

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delta-uvrY versus wild-type

Project description:The BarA/UvrY two-component system is well conserved in numerous species of the γ-proteobacteria. However, the regulatory output of this system differs significantly between the species. In this study, we characterized the BarA/UvrY two-component system in Shewanella oneidensis MR-1. Sensor kinase BarA and the cognate response regulator UvrY were identified by bioinformatic analysis and subsequent in vivo interaction and phosphotransfer studies. The expression of two putative small regulatory RNAs (sRNAs), CsrB1 and CsrB2, was demonstrated to be dependent on UvrY. In contrast, a third predicted sRNA, CsrC, was expressed independently of UvrY. Transcriptomic analysis by microarrays revealed that UvrY is a global regulator and affects transcription of key enzymes of carbon metabolism such as ackA, aceAB, and pflAB. Subsequent growth experiments demonstrated that mutants lacking UvrY have a significant growth advantage over the wild type during aerobic growth on N-acetylglucosamine while under under anaerobic conditions the mutant grew more slowly. This effect was absent during growth with lactate as carbon source or in complex medium. Based on these results, we conclude that, in S.oneidensis MR-1, the global BarA/UvrY regulatory system is involved in central carbon metabolism processes.

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