Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: RNA-seq of Cyanobacterium Anabaena sp. Strain PCC 7120

PROVIDER: PRJNA575071 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10208174_1.fastq.gz	Fastqsanger.gz
	SRR10208174_2.fastq.gz	Fastqsanger.gz
	SRR10208175_1.fastq.gz	Fastqsanger.gz
	SRR10208175_2.fastq.gz	Fastqsanger.gz
	SRR10208176_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Identification of the global transcriptional regulator All3953 (RbcR) in the cyanobacterium Anabaena sp. PCC 7120 by ChIP-Seq analysis

Project description:The whole regulon of the LTTR All3953 was determined at 3 h after Ci deficiency in the cyanobacterium Anabaena sp. PCC 7120 by ChIP-Seq analysis. A TAP-tagged version of the protein was used for the chromatin immunoprecipitation. A total of 142 peaks were found, mainly located in the chromosome of Anabaena.

2015-03-07 | GSE58861 | GEO

Deletion and Complementation of Alr3614 in Anabaena PCC7120

Project description:To examine Alr3614 influence on the expression of Anabaena sp. PCC 7120, we have used customized microarrays to identify genes potentially regulated by this short DNA-binding protein. We compared the expression of an Alr3614-deletion strain with an Alr3614-complementation strain.

2022-05-12 | GSE183629 | GEO

Nostoc sp. PCC 7120 = FACHB-418

Project description:RNA-seq of Cyanobacterium Anabaena sp. Strain PCC 7120

| PRJNA574778 | ENA

Transcriptional Regulation of Anabaena 7120 response to Nitrogen Deprivation

2011-06-22 | GSE26633 | GEO

ChIP analysis unravels an exceptionally wide distribution of binding sites for the NtcA transcription factor in a heterocyst-forming cyanobacterium.

Project description:The CRP-family transcription factor NtcA, universally found in cyanobacteria, was initially discovered as a regulator operating N control. It responds to the N regime signaled by the internal 2-oxoglutarate levels, an indicator of the C to N balance of the cells. NtcA-activated canonical promoters bear an NtcA-consensus binding site (GTAN8TAC) centered at about 41.5 nucleotides upstream from the transcription start point. In this study, we have used chromatin immunoprecipitation followed by high-throughput sequencing to identify the whole regulon of NtcA in cells of the filamentous, heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 after the withdrawal of combined N. NtcA has been found to bind to 2,424 target regions in the genome of Anabaena, which have been ascribed to 2,153 genes. Interestingly, only a small proportion of those are involved in N assimilation and metabolism, and 65 % of the target regions were located intragenically. The NtcA regulon identified here constitutes the largest bacterial regulon described to date. Our results show that NtcA has a much wider role in the physiology of the cell than it has been previously thought, acting both as a global transcriptional regulator and possibly also as a factor influencing the superstructure of chromosome (and plasmids). Cells of Anabaena sp. PCC 7120 subjected to N-withdrawal for 3 h were used to perform chromatin immunoprecipitation with anti-NtcA antibody. The immunoprecipitated material was sequenced. Three ChIPs were performed from two independent sets of Anabaena cells. A sample of total DNA (not subjected to immunoprecipitation) was used as a control (Input sample).

2014-01-28 | E-GEOD-51865 | biostudies-arrayexpress

Anabaena PCC7120 infection with Cyanophage A1

Project description:In order to examine the response of Anabaena sp. PCC 7120 to an infection of the Cyanophage A1, we have used customized microarrays to identify genes potentially up- or downregulated by the infection. For this we compared cell culture before and after infection.

2023-11-01 | GSE183630 | GEO

Transcriptional Regulation of Anabaena 7120 response to Nitrogen Deprivation

Project description:Here, we report the transcriptome of Anabaena sp. strain 7120, a cyanobacterium that forms specialized nitrogen-fixing cells called heterocysts. Our data suggests that cyanobacteria frequently have more complex transcripts than thought, with large 5' UTRs, numerous antisense transcripts, and multiple transcriptional start sites or processing sites. Four samples of total filament RNA were sequenced with Illumina 40bp reads using directional RNA sequencing (see the Illumina small RNA prep protocol). The samples are 0hr (vegetative cells grown in the presence of ammonia) and 6hr, 12hr, and 21hr cells (after nitrogen step down).

2011-06-22 | E-GEOD-26633 | biostudies-arrayexpress

Impaired cell-cell communication in multicellular cyanobacteria affects carbon uptake, photosynthesis and the cell wall

Project description:Cell-cell communication is an essential attribute of multicellular organisms. We studied the effects of perturbed communication in multicellular filaments of mutant derivatives of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, in which septal proteins were deleted. Filaments of sepJ and sepJ/fraC/fraD deleted strains showed marked differences in growth, pigment absorption spectra and spatial patterns of expression of the hetR gene encoding a master regulator of heterocyst differentiation. To understand the global changes in gene expression that take place as a result of impaired cell-cell molecular transfer, we mapped the transcriptional landscape of wild-type and mutant filaments using RNA-seq, both under nitrogen-replete and nitrogen-poor conditions. Our results show that the effects of sepJ and fraC/fraD deletions are not additive, and that far from affecting only passive transport between cells, perturbations to cell-cell communication in this model organism lead to significant changes in gene expression. Most significant effects include increased expression of genes encoding carbon uptake systems and some components of the photosynthetic apparatus, and decreased expression of genes encoding cell wall components related to heterocyst differentiation and to polysaccharide export. Thus, impairment of intercellular communication strongly affects specific aspects, notably related to carbon metabolism, of the biology of Anabaena.

2021-01-19 | GSE161085 | GEO

HILIC high resolution separation for iTRAQ high throughput proteomics

Project description:HILIC runs (separate LC-MS) of mixed proteome from closely related cyanobacterium Nostoc punctiforme ATCC 29133 and Nostoc sp. PCC 7120. Quantitative comparisons across species can only be made using orthologous peptides. All other peptides are used to assess biological variation and MS/MS co-elution study.

2011-03-18 | PRD000374 | Pride

Project description:Anabaena sp. PCC 7120 bacteriophage Elbi

| PRJNA727523 | ENA