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Genome-wide screening of N6-methyladenosine modification pattern in clear cell renal cell carcinoma (ccRCC)

ABSTRACT: Genome-wide screening of N6-methyladenosine modification pattern in clear cell renal cell carcinoma (ccRCC)

PROVIDER: PRJNA575254 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10212364_1.fastq.gz	Fastqsanger.gz
	SRR10212364_2.fastq.gz	Fastqsanger.gz
	SRR10212365_1.fastq.gz	Fastqsanger.gz
	SRR10212365_2.fastq.gz	Fastqsanger.gz
	SRR10212366_1.fastq.gz	Fastqsanger.gz

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Genome-wide screening of N6-methyladenosine modification pattern in clear cell renal cell carcinoma (ccRCC)

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N6-methyladenosine Modulates Messenger RNA Translation Efficiency

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Project description:Oxaliplatin as a first-line drug frequently causes the chemo-resistance on colorectal cancer (CRC). N6-methyladenosine (m6A) methylation has been largely acknowledged in multiple biological functions. However, the molecular mechanisms underlying the m6A methylation in modulating anticancer drug resistance in CRC are still obscure. In present study, RIP-seq was conducted to investigate the occupancy of N6-methyladenosine RNA binding protein 3 (YTHDF3) served as “readers” that can recognize m6A modification site in HCT116 cells with oxaliplatin resistance (HCT116R). Then, YTHDF3 was knockdown by siRNA in HCT116 cells with oxaliplatin resistance, and RIP-seq was further conducted to investigate m6A methylation of HCT116, HCT116R and HCT116R cells with YTHDF3 knockdown.

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Project description:N6-methyladenosine (m6A) is the most abundant internal modification in the messenger RNA (mRNA) of all higher eukaryotes. This modification has been shown to be reversible in mammals; it is installed by a methyltransferase heterodimer complex of METTL3 and METTL14 bound with WTAP, and reversed by iron(II)- and Î±-ketoglutarate-dependent demethylases FTO and ALKBH5. This modification exhibits significant functional roles in various biological processes. The m6A modification as a RNA mark is recognized by reader proteins, such as YTH domain family proteins and HNRNPA2B1; m6A can also act as a structure switch to affect RNA-protein interactions for biological regulation. In Arabidopsis thaliana, the methyltransferase subunit MTA (the plant orthologue of human METTL3, encoded by At4g10760) was well characterized and FIP37 (the plant orthologue of human WTAP) was first identified as the interacting partner of MTA. Here we report the discovery and characterization of reversible m6A methylation mediated by AtALKBH10B (encoded by At4g02940) in A. thaliana, and noticeable roles of this RNA demethylase in affecting plant development and floral transition. Our findings reveal potential broad functions of reversible mRNA methylation in plants. m6A peaks were identified from wild type Columbia-0 and atalkbh10b-1 mutant in two biological replicates

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