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Global transcriptomic analysis of Drosophila Hinfp mutant and wild-type gut to understand their role in genome stability

ABSTRACT: Global transcriptomic analysis of Drosophila Hinfp mutant and wild-type gut to understand their role in genome stability

PROVIDER: PRJNA575851 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10231139.fastq.gz	Fastqsanger.gz
	SRR10231140.fastq.gz	Fastqsanger.gz
	SRR10231141.fastq.gz	Fastqsanger.gz
	SRR10231142.fastq.gz	Fastqsanger.gz
	SRR10231143.fastq.gz	Fastqsanger.gz

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Global transcriptomic analysis of Drosophila Hinfp mutant and wild-type gut to understand their role in genome stability

Project description:Drosophila hinfp mutant is lethal. They are unble to ecolose from the pupal case and died. To investigate the molecular function of Hinfp in Drosophila gut, we isolated RNA from mutant and wild type adult pharate gut and performed RNA-seq analysis. The comparison of mutant and control samples revealed significantly changed expression of over more than thousand Drosophila genes. Among the down regulated genes were many Histone genes. Further validation of RNA-seq data of Histone genes divulged that His1 is significantly down-regulated lead to genome instability through de-repression of menay of the retrotransposon. We also rescued the all phenotypes by overexpression of UAS-Hinfp and UAS-His1 gene.

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