Project description:The complete DNA sequence of the mitochondrial genome of the bullsnake (Pituophis catenifer sayi) is presented and analyzed in this study. The genome is 17,193?bp in length, and contains 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 2 control regions. The overall base composition of the H-strand is A (34.5%), T (26.2%), C (25.8%) and G (12.7%). The gene order and orientation of the mitogenome is consistent with other sequenced genomes from colubrid snakes. Phylogenetic analyses using the ML, NJ and MP methods for a set of colubrids, including every sequenced genus within the sub-family Colubrinae, produced identical trees. We show that Pituophis is most closely related to the American ratsnake genus Pantherophis.
Project description:On the North American Great Plains, several snake species reach their northern range limit where they rely on sparsely distributed hibernacula located in major river valleys. Independent colonization histories for the river valleys and barriers to gene flow caused by the lack of suitable habitat between them may have produced genetically differentiated snake populations. To test this hypothesis, we used 10 microsatellite loci to examine the population structure of two species of conservation concern in Canada: the eastern yellow-bellied racer (Coluber constrictor flaviventris) and bullsnake (Pituophis catenifer sayi) in 3 major river valleys in southern Saskatchewan. Fixation indices (FST) showed that populations in river valleys were significantly differentiated for both species (racers, FST = 0.096, P = 0.001; bullsnakes FST = 0.045-0.157, P = 0.001). Bayesian assignment (STRUCTURE) and ordination (DAPC) strongly supported genetically differentiated groups in the geographically distinct river valleys. Finer-scale subdivision of populations within river valleys was not apparent based on our data, but is a topic that should be investigated further. Our findings highlight the importance of major river valleys for snakes at the northern extent of their ranges, and raise the possibility that populations in each river valley may warrant separate management strategies.
Project description:Porcine 60K BeadChip genotyping arrays (Illumina) are increasingly being applied in pig genomics to validate SNPs identified by re-sequencing or assembly-versus-assembly method. Here we report that more than 98% SNPs identified from the porcine 60K BeadChip genotyping array (Illumina) were consistent with the SNPs identified from the assembly-based method. This result demonstrates that whole-genome de novo assembly is a reliable approach to deriving accurate maps of SNPs.
