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Single cell transcriptional profile of third instar larval wing discs in Drosophila using drop-seq (single cell RNA-seq)

ABSTRACT: Single cell transcriptional profile of third instar larval wing discs in Drosophila using drop-seq (single cell RNA-seq)

PROVIDER: PRJNA576603 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10250631_1.fastq.gz	Fastqsanger.gz
	SRR10250631_2.fastq.gz	Fastqsanger.gz
	SRR10250632_1.fastq.gz	Fastqsanger.gz
	SRR10250632_2.fastq.gz	Fastqsanger.gz
	SRR10250633_1.fastq.gz	Fastqsanger.gz

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Single cell transcriptional profile of third instar larval wing discs in Drosophila using drop-seq (single cell RNA-seq)

Project description:Our study focuses on understanding the early transcriptional changes taking place during the divergence of the adult muscle precursors that give rise to indirect flight muscles and direct flight muscles in Drosophila. We analyzed the heterogenous cell population of the adult muscle precursors by scRNA-seq and build an integrated single-cell reference atlas. We addressed the differences among muscle-type and different cell state during myoblast differentiation. Also, our dataset includes the transcriptional profile of the epithelial cells localized in the presumptive hinge and notum of third instar larval wing discs. In addition we studied the functional relevance of Amalgam in flight muscle development by depleting Ama expression specifically in the adult muscle precursors. We determined the transcriptional changes and perturbations in AMP cell identity upon Ama knockdown.

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

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Transcriptome analysis of control (y w) and Paip1 mutant third instar larval wing imaginal discs

Project description:We report the genome-wide RNA profiling of third instar larval wing imaginal discs upon Paip1 mutation

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Single cell transcriptional profile of third instar larval wing discs in Drosophila

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Single cell transcriptional profile of third instar larval wing discs in Drosophila

Project description:Single cell transcriptional profile of third instar larval wing discs in Drosophila

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Transcriptomic analysis of third instar larval wing imaginal discs of Drosophila melanogaster corto and uL11 mutants

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae mutant for corto (cortoL1/corto420) The reference line was the w1118 genetic background of the mutant lines.

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Project description:Wandering third instar larvae were dissected where the eye disc was separated from the antenna and brain. The tissues were dissociated into single cells and captured using Drop-seq. Single cell libraries were then generated from each cell and finally sequenced.

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Transcriptomic analysis of third instar larva wing imaginal discs deregulating Cyclin G in Drosophila melanogaster

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae expressing Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) under the control of the daugterless-Gal4 ubiquitous driver. The negative control was transgenic flies wearing only the daugterless-Gal4 driver.

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Genome-wide binding of Cyclin G in Drosophila melanogaster third instar larva wing imaginal discs.

Project description:We describe here the genome-wide binding sites of Cyclin G in Drosophila melanogaster wing imaginal discs from third instar female larvae. We used a transgenic line expressing a version Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) and tagged by Myc under the control of the daugterless-Gal4 ubiquitous driver.

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

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2013-05-21 | E-GEOD-47113 | biostudies-arrayexpress

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