Project description:Gene expression profiling reveals a potential role of TCQA in the upregulation of hair pigmentation in mice hair shaft Skin was isolated from mice dorsal part treated with MilliQ and TCQA; Microarray gene expression profiling was conducted.
Project description:Gene expression profiling reveals a potential role of TCQA in neuronal and pigment cell differentiation of hAECs. hAECs were isolated from discarded term placenta and were treated with 20 μM TCQA for seven days. Microarray gene expression profiling was conducted for biological replicates of TCQA-treated (T7) and untreated control cells on day 0 (D0) and day 7 (D7).
Project description:Gene expression profiling reveals a potential effect of minoxdil and TCQA in stimulating hair growth in 2D cultered dermal papilla cells. HFDPCs were human primary cells line, treated with 0,1 µM minoxidil and 10 µM TCQA for 48 h. Microarray gene expression profiling was conducted for three biological replicates
Project description:Gene expression profiling reveals a potential higher effect of minoxdil and TCQA in stimulating hair growth in 3D cultered dermal papilla cells. HFDPCs were human primary cells line, treated with 0,1 µM minoxidil and 10 µM TCQA for 48 h. Microarray gene expression profiling was conducted for three biological replicates
Project description:TCQA promoted differentiation of hNSCs at least toward the neuronal lineage and suggests the possibility of using TCQA to promote neurogenesis. When differentiation is induced by growth factor withdrawal, decreased expression of stemness gene and increased expression of NSC fate-promoting genes can be observed. Therefore, we evaluated the effect of TCQA on global gene expression in hNSCs during differentiation at 24h to elucidate the neurogenesis-promoting effects of TCQA.
Project description:TCQA- and TFQA- treatments in NSCs showed the significant performance on improving neurogenesis compared with no- treatment NSCs. TCQA and TFQA‐ treatments in NSCs also had significant activation of ErbB signaling pathway, AKT and MAPK kinases. Among them, TCQA- treatment upregulated Myc gene when Jun was mediated in TFQA- treatment, which stimulated the functions of enhancing synaptic growth and neuron differentiation. We used microarrays to detail the global programme of gene expression underlying treatment and identified distinct classes of regulated genes during this process.
Project description:Gene expression profiling study of normally-aging SAMR1 mice brain cortex with TCQA treatment We used microarrays to detail the global gene expression of SAMR1 mouse brain with TCQA treatment as a control for the senescence-acCELerated mouse prone 8 (SAMP8) model of aging mice