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High temporal resolution RNA-seq time series of serum-starved T98G human glioblastoma cells responding to serum stimulation

ABSTRACT: High temporal resolution RNA-seq time series of serum-starved T98G human glioblastoma cells responding to serum stimulation

PROVIDER: PRJNA576691 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10253300_1.fastq.gz	Fastqsanger.gz
	SRR10253300_2.fastq.gz	Fastqsanger.gz
	SRR10253301_1.fastq.gz	Fastqsanger.gz
	SRR10253301_2.fastq.gz	Fastqsanger.gz
	SRR10253302_1.fastq.gz	Fastqsanger.gz

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High temporal resolution RNA-seq time series of serum-starved T98G human glioblastoma cells responding to serum stimulation

Project description:The advent of next-generation sequencing revealed extensive transcription outside the boundaries of annotated protein-coding genes, giving rise to tens of thousands of long non-coding RNAs (lncRNAs). The functional relevance of these transcripts remains unclear. Compellingly, lncRNA expression is strongly linked with adjacent protein-coding gene expression, suggesting potential cis-regulatory roles. To investigate whether these regulatory roles exist, we examine in detail the timing of lncRNA expression relative to transcripts of known function. If a causal cis-regulatory relationship exists, lncRNA activation must necessarily precede changes in adjacent target gene expression. Here, we report a high temporal resolution RNA-seq time course of synchronized T98G human glioblastoma cells responding to serum stimulation. Detailed profiling of the expression dynamics of lncRNAs and protein-coding genes in these synchronized transitioning human cells provides insight into the feasibility of broad-scale cis-regulatory roles for lncRNAs.

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