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RNAseq in PMA/Ca2+ ionophore stimulated HeLa cells transfected with negative control siRNA and siRNA against HNRNPU

ABSTRACT: RNAseq in PMA/Ca2+ ionophore stimulated HeLa cells transfected with negative control siRNA and siRNA against HNRNPU

PROVIDER: PRJNA576700 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10254385_1.fastq.gz	Fastqsanger.gz
	SRR10254385_2.fastq.gz	Fastqsanger.gz
	SRR10254386_1.fastq.gz	Fastqsanger.gz
	SRR10254386_2.fastq.gz	Fastqsanger.gz
	SRR10254387_1.fastq.gz	Fastqsanger.gz

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RNAseq in PMA/Ca2+ ionophore stimulated HeLa cells transfected with negative control siRNA and siRNA against HNRNPU

Project description:We sequenced mRNA from cultured HeLa cells transfected with control or HNRNPU siRNA to compare gene expression level in PMA/Ca2+ ionophore stimulated condition

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HNRNPU regulates lncRNAs nuclear localization

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Expression data from HeLa cells transiently transfected with control siRNA or SON siRNA

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Project description:Circular RNA (circRNA) is a type of endogenous single-stranded and covalently closed non-coding RNA that plays emerging roles in tumorigenesis and aggressiveness. However, the functions and underlying mechanisms of circRNAs in regulating gastric cancer progression still remain elusive. We identify circ-hnRNPU, a circRNA derived from exons 5, 6, 7 and 8 of heterogeneous nuclear ribonucleoprotein U (hnRNPU), as a tumor suppressor of gastric cancer progression. To investigate the mechanisms underlying the functions of circ-hnRNPU, we employed the Illumina HiSeq X Ten as a discovery platform to analyze the transcriptome profiling changes of human gastric cancer MKN-45 and AGS cells in response to stable over-expression of circ-hnRNPU. The results showed that stable over-expression of circ-hnRNPU led to altered expression of 112 human mRNAs, including 64 up-regulated and 48 down-regulated genes, in both MKN-45 and AGS cells. Furthermore, we validated the RNA-seq results by real-time RT-PCR with high identity. Overall, our results provided fundamental information about the transcriptomic changes in response to circ-hnRNPU over-expression in human gastric cancer cells, and these findings will help us understand the pathogenesis of cancer progression.

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Project description:SON is a large Ser/Arg (SR)-related protein localized in nuclear speckles. SON siRNA causes defects in mitotic progression and genome instability. We used microarrays to detail the pattern of gene expression after SON knockdown. HeLa cells were grown in 100 mm dishes and transfected with negative control siRNA or SON siRNA (400 pmol). Then, cells were harvested after 66 hours and RNAs were isolated. Microarrays were performed using GeneChip Human Genome U133A 2.0 (Affymetrix).

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