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A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

ABSTRACT: A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

PROVIDER: PRJNA576722 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10255300.fastq.gz	Fastqsanger.gz
	SRR10255301.fastq.gz	Fastqsanger.gz
	SRR10255302.fastq.gz	Fastqsanger.gz
	SRR10255303.fastq.gz	Fastqsanger.gz
	SRR10255304.fastq.gz	Fastqsanger.gz

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Similar Datasets

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [ChIP-seq]

Project description:PRDM14 is a crucial regulator of mouse primordial germ cells (mPGC), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a previous knockdown study indicated that PRDM14 might be dispensable for human germ cell fate. Here, we decided to use inducible degrons for a more rapid and comprehensive PRDM14 depletion. We show that PRDM14 loss results in significantly reduced specification efficiency and an aberrant transcriptome of human PGC-like cells (hPGCLCs) obtained in vitro from human embryonic stem cells (hESCs). Chromatin immunoprecipitation and transcriptomic analyses suggest that PRDM14 cooperates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT signalling and somatic markers. Notably, PRDM14 targets are not conserved between mouse and human, emphasising the divergent molecular mechanisms of PGC specification. The effectiveness of degrons for acute protein depletion is widely applicable in various developmental contexts.

2019-12-08 | GSE138674 | GEO

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]

2019-12-08 | GSE138673 | GEO

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]

Project description:A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]

| PRJNA576723 | ENA

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [ChIP-seq]

Project description:A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [ChIP-seq]

| PRJNA576728 | ENA

A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.

Project description:PRDM14 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a previous knockdown study indicated that PRDM14 might be dispensable for human germ cell fate. Here, we decided to use inducible degrons for a more rapid and comprehensive PRDM14 depletion. We show that PRDM14 loss results in significantly reduced specification efficiency and an aberrant transcriptome of hPGC-like cells (hPGCLCs) obtained in vitro from human embryonic stem cells (hESCs). Chromatin immunoprecipitation and transcriptomic analyses suggest that PRDM14 cooperates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT signalling and somatic markers. Notably, PRDM14 targets are not conserved between mouse and human, emphasising the divergent molecular mechanisms of PGC specification. The effectiveness of degrons for acute protein depletion is widely applicable in various developmental contexts.

| S-EPMC7062732 | biostudies-literature

Single cell microarray analysis of mouse primordial germ cells with Prdm14 mutation.

Project description:Prdm14 is a critical gene for specifying mouse primordial germ cells (PGCs). The changes in expression in mouse PGCs caused by mutations of the Prdm14 gene were investigated at the single-cell level using microarray analysis.

2024-04-17 | GSE233342 | GEO

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-12-08 | GSE138675 | GEO

Single cell microarray analysis of mouse primordial germ cells with Prdm14 mutation.

Project description:Single cell microarray analysis of mouse primordial germ cells with Prdm14 mutation.

| PRJNA975948 | ENA

Expresssion data of Prdm14 KO ESCs expressing each orthologues.

Project description:Gene regulatory networks underlying cellular pluripotency are controlled by a core circuitry of transcription factors, including POU5F1 and NANOG in mammals. However, the evolutionary origin and transformation of pluripotency transcriptional networks have not been elucidated in deuterostomes. PR domain-containing protein 14 (PRDM14) is specifically expressed in pluripotent cells and germ cells, and is required for establishing embryonic stem cells (ESCs) and primordial germ cells (PGCs) in mice. Here, we compared the functions and expression patterns of PRDM14 orthologues within deuterostomes. Amphioxus PRDM14, zebrafish PRDM14, and the combination of sea urchin PRDM14 and CBFA2T compensated for disruption of the mouse Prdm14 gene in terms of maintaining mouse ESC pluripotency. Interestingly, Prdm14 was expressed in motor neurons, but not in germ cells in amphioxus embryos as observed in zebrafish embryos. Therefore, our results suggest that the integration of the PRDM14–CBFA2T complex into the transcriptional network for pluripotency may be essential for stabilizing pluripotency during the early amniote development.

2019-01-29 | GSE112904 | GEO

ETO Family Protein Mtgr1 Mediates Prdm14 Functions in Stem Cell Maintenance

Project description:Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. Structure-guided point mutants abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression.

2016-01-01 | GSE74547 | GEO

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