Project description:The complete and annotated coding sequence and partial noncoding sequence of an Usutu virus genome were sequenced from RNA extracted from a clinical brain tissue sample obtained from a common hill myna (Gracula religiosa), demonstrating close homology with Usutu viruses circulating in Europe.
Project description:BACKGROUND: Extinction, the capacity for an individual to inhibit or diminish maladaptive fear memories, is a critical aspect of fear processing. In humans, weak extinction learning is often observed in anxiety and fear-related disorders, such as PTSD. However, the mechanisms behind regulating extinction and determining individual variability in extinction learning remain poorly understood. METHODS: To investigate the molecular basis of inter-individual and sex-related differences in the ability to extinguish fear, extinction learning was analyzed in inbred wild-type male and female mice. Extinction tests were combined with in vivo profiling of the differential hippocampal transcriptomes associated with weak and strong extinction learning and genetic manipulations to extend our transcriptomic findings. RESULTS: We identified significant sex-dependent and -independent differences in hippocampal gene expression between weak and strong extinction learner animals. Especially surprising was a very high transcriptomic overlap between weak learner males and females, showing upregulation of multiple genes associated with neurotoxic insult and cellular stress, including a major stress regulator, a prion-like Tia1. Viral overexpression of Tia1 in the dorsal hippocampus caused sex-independent dysregulation of microglia and diminished fear extinction learning in animals of both sexes. CONCLUSIONS: Altogether, we demonstrated the brain-based transcriptomic architecture associated with weak versus strong fear extinction learning in male and female mammalian subjects and identified the sex-independent extinction-suppressive role of hippocampal Tia1 upregulation. Our results should help better understand the mechanisms underlying individual, and sex-dependent differences in extinction and inform novel therapeutic targets for pharmacological extinction augmentation strategies in fear-related disorders.
Project description:We uncover a pivotal role for ESRRB in demarcating ESC-specific enhancer units, and propose that ESRRB’s developmentally-regulated extinction precipitates their decommissioning with impact on the rewiring of the pluripotency transcriptional programme upon embryo implantation.
Project description:We uncover a pivotal role for ESRRB in demarcating ESC-specific enhancer units, and propose that ESRRB’s developmentally-regulated extinction precipitates their decommissioning with impact on the rewiring of the pluripotency transcriptional programme upon embryo implantation
