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Tick metagenome

ABSTRACT: Bacterial communities in Ixodes persulcatus ticks from Northeast China

PROVIDER: PRJNA577275 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10259810_1.fastq.gz	Fastqsanger.gz
	SRR10259810_2.fastq.gz	Fastqsanger.gz
	SRR10259811_1.fastq.gz	Fastqsanger.gz
	SRR10259811_2.fastq.gz	Fastqsanger.gz
	SRR10259812_1.fastq.gz	Fastqsanger.gz

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tick metagenome

Project description:Bacterial communities in Ixodes persulcatus ticks from Northeast China

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Early immunologic events at the tick-host interface

Project description:Ixodes species ticks are competent vectors of tick-borne viruses including tick-borne encephalitis and Powassan encephalitis. Tick saliva has been shown to facilitate and enhance viral infection. This likely occurs by saliva-mediated modulation of host responses into patterns favorable for viral infection and dissemination. Because of the rapid kinetics of tick-borne viral transmission, this modulation must occur as early as tick attachment and initiation of feeding. In this study, the gene expression profile of cutaneous bite-site lesions created by uninfected ticks were analyzed at 1, 3, 6, and 12 hours after Ixodes scapularis nymphal tick attachment to discover host pathways or responses potentially important in tick-borne viral establishment. Four milimeter ear biopsies from BALB/cJ mice infested with Ixodes scapularis nymphs were assayed using Affymetrix genechip 430A 2.0 arrays at 1, 3, 6, and 12 hours after infestation during a primary exposure. 3 mice were measured at each time point. Controls were 3 similarly housed but tick-free mice.

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Early immunologic events at the tick-host interface

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Project description:We performed single-nucleus RNA sequencing (snRNA-seq) of skin and blood of persons presenting with naturally acquired, attached Ixodes scapularis ticks.

2023-10-17 | GSE245516 | GEO

Tick-borne Pathogens Induce Differential Expression of Genes Promoting Cell Survival and Host Resistence in Ixodes ricinus Cells

Project description:There has been an emergence and expansion of tick-borne diseases in Europe, Asia and North America in recent years, including Lyme disease, tick-borne encephalitis, and human anaplasmosis. The primary tick vectors implicated are hard ticks of the Ixodes genera. Although much is known about the host response to these bacterial and viral pathogens, there is limited knowledge of the cellular responses to infection within the tick vector. The bacterium Anaplasma phagocytophilum (A. phagocytophilum), is able to bypass apoptotic processes in ticks, enabling infection to proceed. However, the tick cellular responses to infection with the flaviviruses tick-borne encephalitis virus (TBEV) and louping ill virus (LIV), which cause tick-borne encephalitis and louping ill respectively, are less clear. Infection of an Ixodes ricinus (I. ricinus) tick cell line with the viruses LIV and TBEV, and the bacterium A. phagocytophilum, identified activation of common and distinct cellular pathways. In particular, commonly-upregulated genes included those that modulate apoptotic pathways (HSP70), putative anti-pathogen genes (FKBP and XBL1), and genes that influence the tick innate immune response, including selective activation of toll genes. These data provide an insight into potentially key genes involved in the tick cellular response to viral or bacterial infection.

2017-01-01 | GSE85300 | GEO

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