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Comparison of C. glutamicum growing with GABA as carbon source in the presence or absence of (NH4)2SO4.

ABSTRACT: Comparison of C. glutamicum growing with GABA as carbon source in the presence or absence of (NH4)2SO4.

PROVIDER: PRJNA577459 | ENA |

REPOSITORIES: ENA

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Comparison of C. glutamicum growing with GABA as carbon source in the presence or absence of (NH4)2SO4.

Project description:γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid and widespread in nature from microorganisms to plants and animals. DNA microarray analysis revealed that the transcription of gabTDP was strongly increased in C. glutamicum wild type grown with GABA and urea compared to the same strain cultivated with glucose and urea. Remarkably, the presence of ammonia partially inhibited growth on GABA, and the reasons for it were also investigated in this study.

2020-03-25 | GSE138827 | GEO

Comparison of Corynebacterium glutamicum with GABA or glucose as carbon source

2020-03-25 | GSE138828 | GEO

Determination of transcriptional start sites in the presence of GABA for Corynebacterium glutamicum

Project description:In this study, we analyzed the regulation of ƴ-aminobutyrate (GABA) utilization in Corynebacterium glutamicum by the PucR-type transcriptional regulator GabR and by alternative nitrogen and carbon sources.

2020-09-29 | GSE156688 | GEO

Comparison of Corynebacterium glutamicum with GABA or glucose as carbon source

Project description:Comparison of Corynebacterium glutamicum with GABA or glucose as carbon source

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A new metabolic route for the production of gamma-aminobutyric acid in Corynebacterium glutamicum

Project description:Corynebacterium glutamicum shows a great potential for the production of gamma-aminobutyric acid (GABA) from glucose fermentation via putrescine. GABA, a non-protein amino acid widespread in nature, is a component of pharmaceuticals, foods and the biodegradable plastic polyamide 4. Here, the effect of GABA in the growth of C. glutamicum was evaluated. It was estimated that the presence 1.1 M of GABA in the medium reduces the maximum growth rate of C. glutamicum to half. It was also shown that the presence of GABA in the medium negatively affects the growth of C. glutamicum in ethanol as sole carbon source. Furthermore, a new route for the production of GABA in C. glutamicum was established. GABA production from glucose fermentation via putrescine was achieved by plasmid-based overexpression of putrescine transaminase (PatA) and gamma-aminobutyraldehyde dehydrogenase (PatD) in a putrescine production strain. The resultant strain can produce 5.3 ± 0.1 g L-1 of GABA. GABA production was improved by avoiding the formation of N-acetylputrescine and by reducing the amount of nitrogen in CGXII medium. Deletion of the genes responsible for GABA catabolism and GABA re-uptake led to an increase in the GABA production of 21% achieving a titer 8.0 ± 0.3 g L-1 and an increase in the volumetric productivity of 41% reaching a productivity of 0.31 g L-1 h-1, the highest volumetric productivity achieved so far for GABA production in C. glutamicum from glucose fermentation in flasks fermentations. The results obtained hitherto are very promising and competitive compared to the traditional pathway for the production of GABA.

2015-10-22 | GSE74258 | GEO

Determination of transcriptional start sites in the presence of GABA for Corynebacterium glutamicum

Project description:Determination of transcriptional start sites in the presence of GABA for Corynebacterium glutamicum

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Transcriptome analysis of Corynebacterium glutamicum in response to furfural presence

Project description:To understrand the altered global gene expression levels in C. glutamicum wild type in presence of furfural, transcriptome profiling was performed.

2015-01-01 | GSE55516 | GEO

Transcriptome analysis of Corynebacterium glutamicum in response to furfural presence

Project description:To understrand the altered global gene expression levels in C. glutamicum wild type in presence of furfural, transcriptome profiling was performed. Transcriptome profiles of the wild type grown in CgXII medium without furfural and with furfural stresses (each 6.5 mM, 13 mM, and 20 mM) were compared by using the samples taken at the OD600 of 6 (for the control and experiments). Each experiment was performed with a duplicate.

2015-01-01 | E-GEOD-55516 | biostudies-arrayexpress

Transcriptome analysis for cellobise-adpative C. glutamicum strains

Project description:To understrand the altered gene expressions of cellobiose-positive C. glutamicum over cellobiose-negative C. glutamicum

2016-01-24 | GSE65076 | GEO

GABA regulates IL-1β production in macrophages

Project description:Neurotransmitters have been well-documented to determine immune cell fates; however, whether and how γ-amino butyric acid (GABA) shapes the function of innate immune cells is still obscure. Here, we demonstrated that GABA orchestrates macrophage maturation and inflammation. GABA treatment during macrophage maturation inhibits interleukin (IL)-1β production from inflammatory macrophages. Mechanistically, GABA enhances succinate-FAD-lysine demethylase1 (LSD1) signaling to regulate the histone demethylation of Bcl2l11 and Dusp2, lowering the formation of NLRP3-ASC-Caspase-1 complex. Meanwhile, GABA-succinate axis lowers succinylation of mitochondrial proteins to promote mitochondrial oxidative phosphorylation (OXPHOS). We also found that GABA alleviates the LPS-induced sepsis as well as high-fat diet-induced obesity in mice. Our study proves that GABA is potential in lessening the pro-inflammatory macrophage responses associating with metabolic reprogramming and protein succinylation, thus providing a strategy for treating macrophage-related inflammatory diseases.

2022-11-29 | PXD037901 | Pride

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