Project description:Transcriptional profile of snails exposed to irradiated E. paraensei miricidia and four days later challenged with S. mansoni miricidia. Compared to snails exposed to only irradiated E. paraensei miricidia.
Project description:The project is aimed at the identification of conotoxins and conopeptides from the venom of marine cone snails found in the Indian coastal waters. Peptides of novel sequences will be further characterized in terms of structural and physico-chemical properties by NMR spectroscopy and other biophysical methods and will be studied for the abilities to elicit pharmacological responses against cellular targets.
Project description:Transcriptional profile of BS-90 snails injected with a cocktail of four FREP3 specific 27-mer DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Compared to BS-90 snails injected with a cocktail of three GFP specific DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Experiments were done over the course of 49 days. Snails were collected (10each) at 2 and 4 dpe to S. mansoni for comparison.
Project description:Transcriptional profile of snails exposed to irradiated E. paraensei miricidia and four days later challenged with S. mansoni miricidia. Compared to snails exposed to only irradiated E. paraensei miricidia. Each replicate is comprised of 1 individual snail from the specified treatment group. Six replicates of each treatment were analyzed on the Snail oligo array.
Project description:Transcriptional profile of BS-90 snails injected with a cocktail of four FREP3 specific 27-mer DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Compared to BS-90 snails injected with a cocktail of three GFP specific DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Experiments were done over the course of 49 days. Snails were collected (10each) at 2 and 4 dpe to S. mansoni for comparison. Each replicate is comprised of 1 individual snail from the specified treatment group. Ten replicates of each treatment were analyzed on the Snail oligo array.
Project description:Marine cone snails have attracted researchers from all disciplines but early life stages have received limited attention due to difficulties accessing or rearing juvenile specimens. Here, we document the culture of Conus magus from eggs through metamorphosis to reveal dramatic shifts in predatory feeding behaviour between post-metamorphic juveniles and adult specimens. Adult C. magus capture fish using a set of paralytic venom peptides combined with a hooked radular tooth used to tether envenomed fish. In contrast, early juveniles feed exclusively on polychaete worms using a unique “sting-and-stalk” foraging behaviour facilitated by short, unbarbed radular teeth and a distinct venom repertoire that induces hypoactivity in prey. Our results demonstrate how coordinated morphological, behavioural and molecular changes facilitate the shift from worm- to fish-hunting in C. magus, and showcase juvenile cone snails as a rich and unexplored source of novel venom peptides for ecological, evolutionary and biodiscovery studies.
2023-05-11 | PXD042133 | Pride
Project description:ddRAD sequencing of populations of cone snails
