Project description:We evaluated the profile of lncRNA and mRNA expression in control and 50μg/ml DEP treated HBE cells using the Arraystar Human LncRNA Array v3.0 array,7th generation. Our findings implicates that dysregulation of mitochondria invovled mRNAs may play important role in cytotoxicity of DEP. Examination of lncRNA and mRNA expression in control or DEP-treated HBE cells
Project description:We evaluated the profile of lncRNA and mRNA expression in control and 50μg/ml DEP treated HBE cells using the Arraystar Human LncRNA Array v3.0 array,7th generation. Our findings implicates that dysregulation of mitochondria invovled mRNAs may play important role in cytotoxicity of DEP.
Project description:mRNAs and LncRNAs have been implicated in the regulation of a board range of biological processes including development, immunity and inflammation We used mRNA and LncRNA microarray to explore the detailed biological processes and mechanisms underlying matter-induced pulmonary toxicity
Project description:miRNAs have been implicated in the regulation of a broad range of biological processes including development, immunity and inflammation We used miRNA microarray to explore the detailed biological processes and mechanisms underlying matter-induced pulmonary toxicity
Project description:The present study aimed to investigate the human serum proteome wide interaction of aluminium oxide nanoparticles (Al2O3-NPs). Methodologically, the specific interaction of serum proteins with Al2O3-NPs were explored through high resolution proteomic profiling. Here we employed LC-MS/MS based HRMS protocol to explore the list of interacted proteins. Further, novel human serum proteins interacted with Al2O3-NPs and its associated molecular pathways have been illustrated.
Project description:To elucidate Al2O3 NPs role in recovery of soybean under flooding stress, gel-free proteomic technique was used. The proteomic analysis of root including hypocotyl at 2, and 4 days of flooding with 50 ppm Al2O3 NPs leading to subsequent removal as compared to flooding was performed.
Project description:As a well-known phenomenon, total mRNAs poorly correlate to proteins in their abundances as reported. Recent findings calculated with bivariate models suggested even poorer such correlation, whereas focusing on the translating mRNAs (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) subset. In this study, we analysed the relative abundances of mRNAs, RNC-mRNAs and proteins on genome-wide scale, comparing human lung cancer A549 and H1299 cells with normal human bronchial epithelial (HBE) cells, respectively. As discovered, a strong correlation between RNC-mRNAs and proteins in their relative abundances could be established through a multivariate linear model by integrating the mRNA length as a key factor. The R2 reached 0.94 and 0.97 in A549 versus HBE and H1299 versus HBE comparisons, respectively. This correlation highlighted that the mRNA length significantly contributes to the translational modulation, especially to the translational initiation, favoured by its correlation with the mRNA translation ratio (TR) as observed. We found TR is highly phenotype specific, which was substantiated by both pathway analysis and biased TRs of the splice variants of BDP1 gene, which is a key transcription factor of transfer RNAs. These findings revealed, for the first time, the intrinsic and genome-wide translation modulations at translatomic level in human cells at steady-state, which are tightly correlated to the protein abundance and functionally relevant to cellular phenotypes.
Project description:We performed translatome and transcriptome sequencing of A549, H1299 and HBE cells and analyzed the translation ratio (TR) of all genes Total RNA and ribosome-bound RNA were extracted from A549, H1299 and HBE cells, and the polyA+ mRNA was sequenced using Illumina GAIIx and HiSeq-2000. The reads were mapped to RefSeq RNA reference sequences and the expression level were quantified by rpkM calculation.